Overview

  • Product name

    Anti-Creatine kinase B type antibody
    See all Creatine kinase B type primary antibodies
  • Description

    Rabbit polyclonal to Creatine kinase B type
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Creatine kinase B type aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab157816)

  • Positive control

    • This antibody gave a positive signal in Human Brain tissue lysate as well as the following whole cell lysates: HeLa; SHSY5Y; Y79. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab126418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities

    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

Images

  • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: CKB knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab126418 observed at 43 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab126418 was shown to specifically react with CKB in wild-type HAP1 cells. No bands were observed when CKB knockout samples were examined. Wild-type and CKB knockout samples were subjected to SDS-PAGE. Ab126418 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ICC/IF image of ab126418 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126418 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Creatine kinase B type antibody (ab126418) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 4 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa
    Additional bands at: 70 kDa, 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab126418 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab126418 has not yet been referenced specifically in any publications.

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