Anti-Creatine kinase B type antibody (ab151579)
Key features and details
- Rabbit polyclonal to Creatine kinase B type
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Creatine kinase B type antibody
See all Creatine kinase B type primary antibodies -
Description
Rabbit polyclonal to Creatine kinase B type -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus tropicalis -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 16-209 of Human Creatine kinase B type
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Positive control
- NIH-3T3 and H1299 cell lysates; 293T, HeLaS3, HepG2 cell lines; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 396248 Chicken
- Entrez Gene: 516210 Cow
- Entrez Gene: 1152 Human
- Entrez Gene: 12709 Mouse
- Entrez Gene: 24264 Rat
- Omim: 123280 Human
- SwissProt: P05122 Chicken
- SwissProt: Q5EA61 Cow
see all -
Alternative names
- B CK antibody
- B-CK antibody
- BB-CK antibody
see all
Images
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Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution + H1299 cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg
Predicted band size: 43 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Creatine kinase B with ab151579 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded human hepatoma tissue, labeling Creatine kinase B with ab151579 at 1/500 dilution.
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All lanes : Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution
Lane 1 : Xenopus laevis brain tissue lysate - whole
Lane 2 : HEK293 cell lysate
Lane 3 : Rat brain tissue lysate - whole
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/1000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 75-135 kDa (possible non-specific binding)
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151579 has been referenced in 2 publications.
- Samborska B et al. Creatine transport and creatine kinase activity is required for CD8+ T cell immunity. Cell Rep 38:110446 (2022). PubMed: 35235777
- Li C et al. Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis. Int J Biol Macromol 162:11-23 (2020). PubMed: 32531365