Overview

  • Product name

    Anti-Creatine kinase B type antibody
    See all Creatine kinase B type primary antibodies
  • Description

    Rabbit polyclonal to Creatine kinase B type
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human, Xenopus laevis
    Predicted to work with: Rat, Chicken, Cow, Pig, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 16-209 of Human Creatine kinase B type

  • Positive control

    • NIH-3T3 and H1299 cell lysates; 293T, HeLaS3, HepG2 cell lines; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities

    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

Images

  • Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution + H1299 cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution + NIH-3T3 cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Creatine kinase B with ab151579 at 1/200 dilution.
  • Immunohistochemical analysis of paraffin-embedded hepatoma tissue, labeling Creatine kinase B with ab151579 at 1/500 dilution.
  • All lanes : Anti-Creatine kinase B type antibody (ab151579) at 1/1000 dilution

    Lane 1 : Xenopus laevis brain tissue lysate - whole
    Lane 2 : HEK293 cell lysate
    Lane 3 : Rat brain tissue lysate - whole

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (H+L) polyclonal at 1/1000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa
    Additional bands at: above 75 kDa (possible non-specific binding)


    Exposure time: 30 seconds

    See Abreview

References

ab151579 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Xenopus laevis Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 18 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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