Overview

  • Product name
    Anti-Creatine kinase B type antibody
    See all Creatine kinase B type primary antibodies
  • Description
    Rabbit polyclonal to Creatine kinase B type
  • Host species
    Rabbit
  • Specificity
    This antibody is specific for Creatine kinase BB.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A KLH conjugated synthetic peptide selected within aa 200~300 of human Creatine Kinase BB.

  • Positive control
    • Y79 cell lysate and mouse colon tissue lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab38211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

Target

  • Function
    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities
    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • B CK antibody
    • B-CK antibody
    • BB-CK antibody
    • BCK antibody
    • Brain creatine kinase antibody
    • Ckb antibody
    • CKBB antibody
    • Creatine kinase B antibody
    • Creatine kinase B chain antibody
    • Creatine kinase B type antibody
    • Creatine kinase B-type antibody
    • Creatine Kinase BB Isoenzyme antibody
    • Creatine kinase brain antibody
    • Creatine kinase brain type antibody
    • Creatine phosphokinase BB antibody
    • Epididymis luminal protein 211 antibody
    • Epididymis secretory protein Li 29 antibody
    • HEL 211 antibody
    • HEL S 29 antibody
    • KCRB_HUMAN antibody
    see all

Images

  • All lanes : Anti-Creatine kinase B type antibody (ab38211) at 1/100 dilution

    Lane 1 : Y79 cell lysate
    Lane 2 : Mouse colon tissue lysate

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

  • Anti-Creatine kinase B type antibody (ab38211) at 1/250 dilution + Tissue lysate prepared from human brain tissue at 10 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/7500 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa


    Exposure time: 1 minute

    See Abreview

References

This product has been referenced in:
  • Mochel F  et al. Early alterations of brain cellular energy homeostasis in huntington disease models. J Biol Chem 287:1361-70 (2012). WB ; Mouse . Read more (PubMed: 22123819) »
  • Zhang SF  et al. Impaired brain creatine kinase activity in Huntington's disease. Neurodegener Dis 8:194-201 (2011). Mouse . Read more (PubMed: 21124007) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us.

I have checked the following alignments with the following homology results:
CK BB: human-mouse: 97%
CK MM: human-mouse: 97%
CK MB: human-mouse: 97% - there is no separate sequence for MB as the protein is a heterodimer of the M and the B chain.

CKMM is the major form in skeletal muscle and myocardium, MB exists in myocardium, and BB exists in many tissues, especially brain.


I used the following sequences from SwissProt:
human CK BB: http://www.uniprot.org/uniprot/P12277
human CK MM: http://www.uniprot.org/uniprot/P06732
mouse CK BB: http://www.uniprot.org/uniprot/Q04447
mouse CK MM: http://www.uniprot.org/uniprot/P07310


As for the homology between human CK BB and MM: 81%
Regions of low homologies are between residues 8-20,162-173,254-272 , 361-381

As for the homology between mouse CK BB and MM: 79%
Regions of low homologies are between residues 1-21, 162-177, 254-271, 299-314, 359-379

Thus, it would be importantto find antibodies that were raised againstone of these regions in order to avoid crossreactivity. As for the MB form,I am not certain if the antibodies raised against the MB form would be absolutely specific- based on the nature of the protein being composed of the B and M chain.


For creatine kinase B, we have 5 antibodies: ab38211, ab92452, ab108388, ab125114,and ab38212 that were tested in WB and mouse.

For ab92452, we know that the immunogen maps at the C-terminus. For ab125114, we also know the immunogen peptide as residues 4-20. Both these antibodies would be a good choice for low crossreactivity.


For creatine kinase M, we have 1 antibody (ab126244) thatis predicted to work in mouse, all other antibodies have only been tested in human. For ab126244, the immunogen was a fragment (residues 1-328), thus we do not know where the antibody would bind.


For creatine kinaseMB, we have 2 antibodies that have been tested in WB but not yet in mouse: ab31832 and ab72002.
ab31832 was raised against the full length protein, thsu we donot know where the antibody would bind, and ab72002 was raised againsta recombinant human Creatine Kinase MB protein purified from E.coli, thus, again we would not know the binding site.


If you wanted to try any of the antibodies that were tested only in human so far -in mouse, you would be eligible to take advantage of our testing dicount program. See below for more details.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - other (Brain)
Loading amount
10 µg
Specification
Brain
Treatment
homogenisation in guanidine thiocyanate with subsequent ultracentrifugation over caesium chloride and dialysis
Gel Running Conditions
Reduced Denaturing (4-20%, 1mm)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 02 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (brain)
Loading amount
5 µg
Specification
brain
Gel Running Conditions
Reduced Denaturing (4-12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Feb 14 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up