Anti-Creatine kinase B type antibody [EPR3927] (ab92452)
- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Creatine kinase B type antibody [EPR3927]
See all Creatine kinase B type primary antibodies -
DescriptionRabbit monoclonal [EPR3927] to Creatine kinase B type
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Creatine kinase B type aa 350-450 (C terminal). The exact sequence is proprietary.
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Positive control
- SHSY-5Y, HeLa, Y-79 and SW840 cell lysate. Human glioma tissue.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR3927
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab92452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Predicted molecular weight: 43 kDa. | |
| IHC-P | 1/100 - 1/500. Antigen retrieval and the use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining. |
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| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/100 - 1/500. |
Target
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FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
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Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 1152 Human
- Entrez Gene: 12709 Mouse
- Entrez Gene: 24264 Rat
- Omim: 123280 Human
- SwissProt: P12277 Human
- SwissProt: Q04447 Mouse
- SwissProt: P07335 Rat
- Unigene: 173724 Human
see all -
Alternative names
- B CK antibody
- B-CK antibody
- BB-CK antibody
see all
Images
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![Western blot - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)](https://www.abcam.com/ps/products/92/ab92452/Images/ab92452-266727-ab92452KOWB.jpg)
Western blot - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Creatine kinase B knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92452 observed at 45 kDa. Red - loading control, ab18058, observed at 2X kDa.ab92452 was shown to specifically react with Creatine kinase B when ProteinX knockout samples were used. Wild-type and Creatine kinase B knockout samples were subjected to SDS-PAGE. ab92452 and ab18058 (loading control to vinculin) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)](https://www.abcam.com/ps/products/92/ab92452/Images/Creatine-Kinase-BB-Primary-antibodies-ab92452-2.jpg)
Western blot - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)All lanes : Anti-Creatine kinase B type antibody [EPR3927] (ab92452) at 1/10000 dilution
Lane 1 : SHSY-5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Y-79 cell lysate
Lane 4 : SW840 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 43 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)](https://www.abcam.com/ps/products/92/ab92452/Images/Creatine-Kinase-BB-Primary-antibodies-ab92452-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)Paraffin embedded Human glioma tissue labelled with ab92452 at 1/100 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)](https://www.abcam.com/ps/products/92/ab92452/Images/Creatine-Kinase-BB-Primary-antibodies-ab92452-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)Immunofluorescence analysis of HeLa cells labelled with ab92452 at 1/100 dilution. -
![Flow Cytometry - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)](https://www.abcam.com/ps/products/92/ab92452/Images/Creatine-kinase-B-type-Primary-antibodies-ab92452-5.jpg)
Flow Cytometry - Anti-Creatine kinase B type antibody [EPR3927] (ab92452)Overlay histogram showing HeLa cells stained with ab92452 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92452, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
References
ab92452 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"