Anti-Creatine Kinase MB antibody [BDI937] (ab19603)

Reviews (1)Q&A (5)References (1)

Overview

  • Product name
    Anti-Creatine Kinase MB antibody [BDI937]
    See all Creatine Kinase MB primary antibodies
  • Description
    Mouse monoclonal [BDI937] to Creatine Kinase MB
  • Host species
    Mouse
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified Human Creatine Kinase MB

Properties

Applications

Our Abpromise guarantee covers the use of ab19603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Creatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.
  • Cellular localization
    Cytoplasmic and Mitochondrial
  • Database links
    • Alternative names
      • B-CK antibody
      • brain creatine kinase antibody
      • CKBB antibody
      • CKMB antibody
      • CKMM antibody
      • creatine kinase B antibody
      • creatine kinase brain antibody
      • creatine kinase M antibody
      • Creatine kinase MB antibody
      • creatine kinase muscle antibody
      • M-CK antibody
      • muscle creatine kinase antibody
      see all

    References

    This product has been referenced in:
    • Garay F  et al. Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples. Anal Bioanal Chem 397:1873-81 (2010). Read more (PubMed: 20449577) »
    See 1 Publication for this product

    Publishing research using ab19603? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-6 of 6 Abreviews or Q&A

    ELISA abreview for Anti-Creatine Kinase MB antibody [BDI937]

     Excellent
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Purified protein (Purified native Human CKMB protein)
    Specification
    Purified native Human CKMB protein
    Type
    Sandwich (Capture)
    Read More

    Mr. Chang Su Jeon

    Verified customer

    Submitted Jul 27 2015

    Question

    Thank you for your reply.
    I informed your reply to the customer but she would like to know more detail information of ab19603, ab72002. And ab405 is discontinued so she couldn’t confirm. Please let me know if you have information.

    Best regards

    Read More
    Answer

    Thank you for your response.

    As the datasheets indicates here are the immunogens used for the following antibodies:

    ab19603 - Purified Human Creatine Kinase MB,

    ab72002 - Recombinant human Creatine Kinase MB protein purified from E.coli

    ab405 - Full length native protein (purified) (Human),

    I regret to inform you that the originator of this antibody no longer has sufficient stocks for us to be able to make this product available to our customers. We have therefore been forced to withdraw ab405 from our product range.

    I do hope that this does not cause you too much inconvenience.

    Read More

    Question

    Dear technical team,

    Our customer purchased ab19603,ab72002,ab405,ab406 and would like to knowimmunogen sequence.
    If you can provide information, please let me know.

    Best regards

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    The immonogen sequence can be found under Immunogen section on the on-line product datasheets. Please advice your customers to visit the website and look through the information provided.

    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Question

    Our customer is going to test sELISA using ab19603-ab110655 or ab19603-ab87580 pair.
    He needs standard protein and let me know if ab73649 or ab72650 is okay. Please let me know which one is better choice.
    Best regards

    Read More
    Answer

    Thank you for contacting us.

    The protein ab73649 is Creatine Kinase MB Isoenzyme Type-I and protein ab73650 is Creatine Kinase MB, type 2. Theoratically both will work; customer can buy any of these.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear technical team,

    Our customer is going to sELISA with Creatine Kinase MB antibodies.
    He wants full length immunogen and I searched ab11045 and ab404 as a monoclonal antibody.
    Let me know which one is meet the customer's needs.

    Best regards,

    Read More
    Answer

    Thank you for your enquiries and your interest.

    Currently, we only have two antibodies (ab19603 and ab110655) which have been tested for sELISA - and only ab19603 is monoclonal.

    Full length proteins are the followings: ab73649 and 73650; they have been tested in ELISA. Please could you and your customer check the on-line datasheets to see if they are suitable for the assay.

    I hope this will be useful for you.

    Read More

    Question

    Our customer raises an enquiry about ab109675 (Creatine Kinase MB protein), ab73649 (Creatine Kinase MB protein (Active)) and ab73650 (Creatine Kinase MB protein (Active)), this customer wants to conduct the ELISA assay with human sample, therefore, she would like to ask for your help to choice any suitable capture antibody and detection antibody with these protein. Could you please help this customer to offer any selection? Thanks for your kindly help. Best regards

    Read More
    Answer

    Thank you for contacting us. The tested pair of anti Creatine Kinase MB is ab19603 - ab110655. Please be advised that none of the two antibodies is conjugated so you may need a tertiary conjugated antibody for detection in sELISA. We suggest using mouse mono as capture and polyclonal as detection antibody. Theoretically the proteins ab73649, ab73650, and ab109675 could be used as positive control however we have not particularly tested these antibodies together so we will not be able to guarantee it,. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up