• Product name

    Anti-Creatine Kinase MB antibody [CK1]
    See all Creatine Kinase MB primary antibodies
  • Description

    Mouse monoclonal [CK1] to Creatine Kinase MB
  • Host species

  • Specificity

    CK1 recognises human creatine kinase MB isoenzyme. Its affinity for the creatine kinase BB isoenzyme is greater than its affinity for creatine kinase MB isoenzyme. hCK-MB - 100% hCK-BB - 182% hCK-MM - 7.3% Affinity constant: 2x109 l/mol human CK-MB.
  • Tested applications

    Suitable for: ELISA, RIAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human).



Our Abpromise guarantee covers the use of ab404 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.


  • Relevance

    Creatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.
  • Cellular localization

    Cytoplasmic and Mitochondrial
  • Database links

  • Alternative names

    • B-CK antibody
    • brain creatine kinase antibody
    • CKBB antibody
    • CKMB antibody
    • CKMM antibody
    • creatine kinase B antibody
    • creatine kinase brain antibody
    • creatine kinase M antibody
    • Creatine kinase MB antibody
    • creatine kinase muscle antibody
    • M-CK antibody
    • muscle creatine kinase antibody
    see all


This product has been referenced in:

  • Garay F  et al. Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples. Anal Bioanal Chem 397:1873-81 (2010). Read more (PubMed: 20449577) »
  • Crawford RM  et al. Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo. FASEB J 16:102-4 (2002). Read more (PubMed: 11729098) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A


Thank you for your enquiry. I can confirm the pI range for ab404 Creatine Kinase MB antibody [CK1] is 6.9 to 8.3. Antibodies have ranges of pI rather than points. I hope this information is helpful to you. Should you have any further questions, please do not hesitate to contact us.

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All the information we have on species cross reactivity is specified on the datasheet. To our knowledge, cross reactivity with mouse has not yet been tested. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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This antibody has not been tested in immunoprecipitation, so we can't guarantee that it will work.

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Dear Gary Please see my answer to your question on ab8364. This is essentially repeated below. This antibody hasn't been tested in Westerns. It is a monoclonal and it works in ELISA and Radioimmunoassays. It is possible that it works in Westerns and I would expect it to work in native gels, however the epitope it recognises may be dependent on tertiary structure and if this is the case it probably wouldn't work in denaturing gels. In general, I believe that ELISA and Radioimmunoassays are performed using native protein. I hope this helps. Regards Michael

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This antibody has not been tested in Western blotting applications, but a good starting dilution for both ELISA and Western would be 1/1000.

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This antibody has not been tested for cross-reactivity against mouse. However as it was raised in mouse, cross-reactivity against the mouse antigen is unlikely.

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