Key features and details
- Mouse monoclonal [CK1] to Creatine Kinase MB
- Suitable for: ELISA, RIA
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Creatine Kinase MB antibody [CK1]
See all Creatine Kinase MB primary antibodies
DescriptionMouse monoclonal [CK1] to Creatine Kinase MB
SpecificityCK1 recognises human creatine kinase MB isoenzyme. Its affinity for the creatine kinase BB isoenzyme is greater than its affinity for creatine kinase MB isoenzyme. hCK-MB - 100% hCK-BB - 182% hCK-MM - 7.3% Affinity constant: 2x109 l/mol human CK-MB.
Tested applicationsSuitable for: ELISA, RIAmore details
Species reactivityReacts with: Human
Full length native protein (purified) (Human).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 6.00
Preservative: 0.095% Sodium azide
Constituents: 0.335% PBS, 1.0878% Sodium citrate, 0.9% Sodium chloride
Concentration information loading...
PurityProtein A purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
RelevanceCreatine Kinase MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. Creatine Kinase MB reversibly catalyses the transfer of phosphate between ATP and various phosphogens. The creatine kinase isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands such as skeletal muscle, heart, brain and spermatozoa.
Cellular localizationCytoplasmic and Mitochondrial
- B-CK antibody
- brain creatine kinase antibody
- CKBB antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab404 has been referenced in 2 publications.
- Garay F et al. Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples. Anal Bioanal Chem 397:1873-81 (2010). PubMed: 20449577
- Crawford RM et al. Creatine kinase is physically associated with the cardiac ATP-sensitive K+ channel in vivo. FASEB J 16:102-4 (2002). PubMed: 11729098