Overview

  • Product name

    Anti-Creatine Kinase MM antibody
    See all Creatine Kinase MM primary antibodies
  • Description

    Rabbit polyclonal to Creatine Kinase MM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1 and 328 of Human Creatine Kinase MM (NP_001815).

  • Positive control

    • HCT116 cell lysate and HeLa whole cell lysate (ab150035)

Properties

Applications

Our Abpromise guarantee covers the use of ab126244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.

Target

  • Function

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
  • Sequence similarities

    Belongs to the ATP:guanido phosphotransferase family.
    Contains 1 phosphagen kinase C-terminal domain.
    Contains 1 phosphagen kinase N-terminal domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • CKM antibody
    • CKMM antibody
    • Creatine kinase M antibody
    • Creatine kinase M chain antibody
    • Creatine kinase M type antibody
    • Creatine kinase M-type antibody
    • Creatine kinase muscle antibody
    • Creatine kinase, muscle type antibody
    • KCRM_HUMAN antibody
    • M-CK antibody
    • MCK antibody
    • Muscle creatine kinase antibody
    see all

Images

  • Anti-Creatine Kinase MM antibody (ab126244) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 43 kDa



    10% SDS PAGE

References

This product has been referenced in:

  • Ma X  et al. Dietary L-arginine supplementation affects the skeletal longissimus muscle proteome in finishing pigs. PLoS One 10:e0117294 (2015). WB ; Pig . Read more (PubMed: 25635834) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

I have checked the following alignments with the following homology results:
CK BB: human-mouse: 97%
CK MM: human-mouse: 97%
CK MB: human-mouse: 97% - there is no separate sequence for MB as the protein is a heterodimer of the M and the B chain.

CKMM is the major form in skeletal muscle and myocardium, MB exists in myocardium, and BB exists in many tissues, especially brain.


I used the following sequences from SwissProt:
human CK BB: http://www.uniprot.org/uniprot/P12277
human CK MM: http://www.uniprot.org/uniprot/P06732
mouse CK BB: http://www.uniprot.org/uniprot/Q04447
mouse CK MM: http://www.uniprot.org/uniprot/P07310


As for the homology between human CK BB and MM: 81%
Regions of low homologies are between residues 8-20,162-173,254-272 , 361-381

As for the homology between mouse CK BB and MM: 79%
Regions of low homologies are between residues 1-21, 162-177, 254-271, 299-314, 359-379

Thus, it would be importantto find antibodies that were raised againstone of these regions in order to avoid crossreactivity. As for the MB form,I am not certain if the antibodies raised against the MB form would be absolutely specific- based on the nature of the protein being composed of the B and M chain.


For creatine kinase B, we have 5 antibodies: ab38211, ab92452, ab108388, ab125114,and ab38212 that were tested in WB and mouse.

For ab92452, we know that the immunogen maps at the C-terminus. For ab125114, we also know the immunogen peptide as residues 4-20. Both these antibodies would be a good choice for low crossreactivity.


For creatine kinase M, we have 1 antibody (ab126244) thatis predicted to work in mouse, all other antibodies have only been tested in human. For ab126244, the immunogen was a fragment (residues 1-328), thus we do not know where the antibody would bind.


For creatine kinaseMB, we have 2 antibodies that have been tested in WB but not yet in mouse: ab31832 and ab72002.
ab31832 was raised against the full length protein, thsu we donot know where the antibody would bind, and ab72002 was raised againsta recombinant human Creatine Kinase MB protein purified from E.coli, thus, again we would not know the binding site.


If you wanted to try any of the antibodies that were tested only in human so far -in mouse, you would be eligible to take advantage of our testing dicount program. See below for more details.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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