Key features and details
- Goat polyclonal to Creatine Kinase MM
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Creatine Kinase MM antibody
See all Creatine Kinase MM primary antibodies
DescriptionGoat polyclonal to Creatine Kinase MM
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken, Cow, Dog, Pig
- WB: Human and mouse heart lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab174672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.
- Information by UniProt
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
All lanes : Anti-Creatine Kinase MM antibody (ab174672) at 0.03 µg/ml
Lane 1 : Human heart lysate (in RIPA buffer)
Lane 2 : Mouse heart lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 43 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab174672 has been referenced in 1 publication.
- Lynch JM et al. Mutant myocilin impacts sarcomere ultrastructure in mouse gastrocnemius muscle. PLoS One 13:e0206801 (2018). PubMed: 30395621