Overview

  • Product name
    Anti-Creatine Kinase MM antibody
    See all Creatine Kinase MM primary antibodies
  • Description
    Goat polyclonal to Creatine Kinase MM
  • Host species
    Goat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human, Pig
    Predicted to work with: Rat, Rabbit, Chicken, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human Creatine Kinase MM aa 258-270 (internal sequence) (Cysteine residue). (NP_001815.2)
    Sequence:

    QKIEEIFKKAGHP


    Database link: P06732

  • Positive control
    • WB: Human, mouse and pig heart lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab174672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).

Target

Images

  • All lanes : Anti-Creatine Kinase MM antibody (ab174672) at 0.03 µg/ml

    Lane 1 : Human heart lysate (in RIPA buffer)
    Lane 2 : Mouse heart lysate (in RIPA buffer)

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 45 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

  • Anti-Creatine Kinase MM antibody (ab174672) at 0.3 µg/ml + Pig heart lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 43 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Primary incubation was 1 hour.

References

ab174672 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rabbit Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
35 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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