Western blot abreview for Anti-Creatine Kinase MM antibody

Excellent
Abreviews
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
35 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS Tween
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-Goat IgG Antibody, HRP conjugate
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 45 kDa Non-specific: 39 kDa
Positive control
mouse heart lysate (whole tissue)

Additional data

Additional Notes
1 - 4: rabbit muscle lysate (whole tissue) 5: mouse heart lysate (whole tissue)

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Submitted Oct 30 2017

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