Anti-Creatine Kinase MM antibody (ab233201)
Key features and details
- Rabbit polyclonal to Creatine Kinase MM
- Suitable for: IHC-P, WB
- Reacts with: Pig
- Isotype: IgG
Overview
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Product name
Anti-Creatine Kinase MM antibody
See all Creatine Kinase MM primary antibodies -
Description
Rabbit polyclonal to Creatine Kinase MM -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Pig
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human -
Immunogen
Recombinant fragment corresponding to Pig Creatine Kinase MM aa 1 to the C-terminus.
Database link: Q5XLD3 -
Positive control
- IHC-P: Pic cardiac muscle and skeletal muscle tissue. WB: Recombinant pig Creatine Kinase MM protein; Pig brain, heart and muscle lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 43 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 43 kDa. |
Target
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 476435 Dog
- Entrez Gene: 1158 Human
- Entrez Gene: 12715 Mouse
- Entrez Gene: 24265 Rat
- Omim: 123310 Human
- SwissProt: P05123 Dog
- SwissProt: P06732 Human
- SwissProt: P07310 Mouse
see all -
Alternative names
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
see all
Images
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All lanes : Anti-Creatine Kinase MM antibody (ab233201) at 3 µg/ml
Lane 1 : Pig brain tissue lysate.
Lane 2 : Pig heart tissue lysate.
Lane 3 : Pig muscle tissue lysate.
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 43 kDa -
Anti-Creatine Kinase MM antibody (ab233201) at 2 µg/ml + Recombinant pig Creatine Kinase MM protein.
Predicted band size: 43 kDa -
Formalin-fixed, paraffin-embedded Pig skeletal muscle tissue stained for Creatine Kinase MM using ab233201 at 20ug/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded Pig cardiac muscle tissue stained for Creatine Kinase MM using ab233201 at 20ug/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab233201 has not yet been referenced specifically in any publications.