Key features and details
- Rabbit polyclonal to Creatine Kinase MM
- Suitable for: IHC-P, WB
- Reacts with: Pig
- Isotype: IgG
Product nameAnti-Creatine Kinase MM antibody
See all Creatine Kinase MM primary antibodies
DescriptionRabbit polyclonal to Creatine Kinase MM
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Pig
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Human
Recombinant fragment corresponding to Pig Creatine Kinase MM aa 11-367.
MPFGNTHNKYKLNFKAEEEYPDLSKHNNHMAKALTLEIYKKLRDKETPSG FTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYVVFKDLFDPIIQDRHGGY KPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGE RRAVEKLSVEALNSLTGEFKGKYYPLKSMTEQEQQQLIDDHFLFDKPVSP LLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVF RRFCVGLQKIEEIFKKAGHPFMWNEHLGYVLTCPSNLGTGLRGGVHVKLA HLSKHPKFEEILTRLRLQKRGTGGVDTAAVGSVFDVSNADRLGSSEVEQV QLVVDGVKLMVEMEKKL
Database link: Q5XLD3
- IHC-P: Pic cardiac muscle and skeletal muscle tissue. WB: Recombinant pig Creatine Kinase MM protein; Pig brain, heart and muscle lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 43 kDa.|
FunctionReversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Sequence similaritiesBelongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain.
- Information by UniProt
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
All lanes : Anti-Creatine Kinase MM antibody (ab233201) at 3 µg/ml
Lane 1 : Pig brain tissue lysate.
Lane 2 : Pig heart tissue lysate.
Lane 3 : Pig muscle tissue lysate.
All lanes : HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 43 kDa
Anti-Creatine Kinase MM antibody (ab233201) at 2 µg/ml + Recombinant pig Creatine Kinase MM protein.
Predicted band size: 43 kDa
Formalin-fixed, paraffin-embedded Pig skeletal muscle tissue stained for Creatine Kinase MM using ab233201 at 20ug/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded Pig cardiac muscle tissue stained for Creatine Kinase MM using ab233201 at 20ug/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233201 has not yet been referenced specifically in any publications.