Anti-Creatine Kinase MM antibody - N-terminal (ab174635)
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Overview
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Product name
Anti-Creatine Kinase MM antibody - N-terminal
See all Creatine Kinase MM primary antibodies -
Description
Goat polyclonal to Creatine Kinase MM - N-terminal -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Dog
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Immunogen
Synthetic peptide corresponding to Human Creatine Kinase MM aa 5-18 (N terminal) (Cysteine residue).
Sequence:NTHNKFKLNYKPEE-C
Database link: P06732 -
Positive control
- WB: Human and mouse skeletal muscle lysate, Human and mouse Heart lysate IHC-P: Human Heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab174635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 476435 Dog
- Entrez Gene: 1158 Human
- Entrez Gene: 12715 Mouse
- Entrez Gene: 24265 Rat
- Omim: 123310 Human
- SwissProt: P05123 Dog
- SwissProt: P06732 Human
- SwissProt: P07310 Mouse
see all -
Alternative names
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine Kinase MM antibody - N-terminal (ab174635)ab174635 (5 µg/ml) staining Creatine Kinase MM in paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot - Anti-Creatine Kinase MM antibody - N-terminal (ab174635)All lanes : Anti-Creatine Kinase MM antibody - N-terminal (ab174635) at 0.00999999977648258 µg
Lane 1 : Human Skeletal Muscle tissue lysate
Lane 2 : Mouse Skeletal Muscle tissue lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Mouse heart tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa
Protocols
Datasheets and documents
References
ab174635 has not yet been referenced specifically in any publications.
