Anti-Creatine Kinase MM antibody - N-terminal (ab174635)
Key features and details
- Goat polyclonal to Creatine Kinase MM - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Creatine Kinase MM antibody - N-terminal
See all Creatine Kinase MM primary antibodies -
Description
Goat polyclonal to Creatine Kinase MM - N-terminal -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Dog
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Immunogen
Synthetic peptide corresponding to Human Creatine Kinase MM aa 5-18 (N terminal) (Cysteine residue).
Sequence:NTHNKFKLNYKPEE-C
Database link: P06732 -
Positive control
- WB: Human and mouse skeletal muscle lysate, Human and mouse Heart lysate IHC-P: Human Heart tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab174635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. -
Sequence similarities
Belongs to the ATP:guanido phosphotransferase family.
Contains 1 phosphagen kinase C-terminal domain.
Contains 1 phosphagen kinase N-terminal domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 476435 Dog
- Entrez Gene: 1158 Human
- Entrez Gene: 12715 Mouse
- Entrez Gene: 24265 Rat
- Omim: 123310 Human
- SwissProt: P05123 Dog
- SwissProt: P06732 Human
- SwissProt: P07310 Mouse
see all -
Alternative names
- CKM antibody
- CKMM antibody
- Creatine kinase M antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Creatine Kinase MM antibody - N-terminal (ab174635)
ab174635 (5 µg/ml) staining Creatine Kinase MM in paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Western blot - Anti-Creatine Kinase MM antibody - N-terminal (ab174635)All lanes : Anti-Creatine Kinase MM antibody - N-terminal (ab174635) at 0.00999999977648258 µg
Lane 1 : Human Skeletal Muscle tissue lysate
Lane 2 : Mouse Skeletal Muscle tissue lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Mouse heart tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 43 kDa
Datasheets and documents
References (0)
ab174635 has not yet been referenced specifically in any publications.
