Product nameAnti-Creatine kinase MT antibody
See all Creatine kinase MT primary antibodies
DescriptionRabbit polyclonal to Creatine kinase MT
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Mouse, Human
A KLH conjugated synthetic peptide selected within aa 1-100 of human CKMT1 (creatine kinase, mitochondrial 1A).
- Mouse colon tissue lysate and ZR-75-1 cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab38278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500. Detects a band of approximately 47, 52 kDa (dependent of cell lysate) (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCreatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Creatine kinase MT occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.
Cellular localizationCell Membrane and Mitochondrial
- Acidic type mitochondrial creatine kinase antibody
- CKMT antibody
- CKMT1 antibody
All lanes : Anti-Creatine kinase MT antibody (ab38278) at 1/100 dilution
Lane 1 : mouse colon tissue lysate
Lane 2 : ZR-75-1 cell lysate
Predicted band size: 47 kDa
Observed band size: 47 and 52 kDa why is the actual band size different from the predicted?
ab38278 has not yet been referenced specifically in any publications.