Anti-Creatine kinase MT (phospho Y153) antibody (ab228517)
Key features and details
- Rabbit polyclonal to Creatine kinase MT (phospho Y153)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-Creatine kinase MT (phospho Y153) antibody
See all Creatine kinase MT primary antibodies -
Description
Rabbit polyclonal to Creatine kinase MT (phospho Y153) -
Host species
Rabbit -
Specificity
Specific for endogenous levels of the ~46 kDa Creatine Kinase MT protein phosphorylated at Tyr153. Immunolabeling of Creatine Kinase MT is greatly decreased by lambda phosphatase treatment. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Sheep, Rabbit, Guinea pig, Hamster, Cow, Dog, Human
-
Immunogen
Synthetic peptide corresponding to Human Creatine kinase MT (phospho Y153).
Database link: P12532 -
Positive control
- WB: Mouse mitochondria from whole brain lysate.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.50
Constituents: 0.24% HEPES, 0.87% Sodium chloride, 0.01% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab228517 is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab228517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 47 kDa. |
Target
-
Relevance
Creatine kinase MT is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Creatine kinase MT occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase, this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. -
Cellular localization
Cell Membrane and Mitochondrial -
Database links
- Entrez Gene: 281692 Cow
- Entrez Gene: 478277 Dog
- Entrez Gene: 548596 Human
- Entrez Gene: 12716 Mouse
- Entrez Gene: 100328683 Rabbit
- Entrez Gene: 29593 Rat
- Entrez Gene: 101108520 Sheep
- Omim: 123290 Human
see all -
Alternative names
- Acidic type mitochondrial creatine kinase antibody
- CKMT antibody
- CKMT1 antibody
see all
Images
-
Western blot - Anti-Creatine kinase MT (phospho Y153) antibody (ab228517)All lanes : Anti-Creatine kinase MT (phospho Y153) antibody (ab228517) at 1/1000 dilution
Lane 1 : Mouse mitochondria from whole brain lysate
Lane 2 : Mouse mitochondria from whole brain lysate treated with lambda phosphatase (1200 units for 30 minutes)
Predicted band size: 47 kDaPhosphospecificity is shown in Lane 2 where the immunolabeling is greatly decreased by blot treatment with lambda phosphatase (1200 units for 30 minutes).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab228517 has not yet been referenced specifically in any publications.
