Anti-Creatinine antibody (ab30719)

Submit a review Q&A (4)References (1)

Key features and details

  • Sheep polyclonal to Creatinine
  • Suitable for: Competitive ELISA
  • Reacts with: Species independent
  • Isotype: IgG

Overview

  • Product name

    Anti-Creatinine antibody

  • Description

    Sheep polyclonal to Creatinine

  • Host species

    Sheep

  • Tested applications

    Suitable for: Competitive ELISAmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Creatinine(N)-BSA

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Associated products

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab30719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA
1/6000.
Notes
Competitive ELISA
1/6000.

Target

  • Relevance

    Creatinine is a waste product from protein in the diet and from the muscles of the body. Creatinine is removed from the body by the kidneys; as kidney disease progresses, the level of creatinine in the blood increases.

  • Cellular localization

    Secreted

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab30719? Please let us know so that we can cite the reference in this datasheet.

ab30719 has been referenced in 1 publication.

  • Cartland SP  et al. TRAIL deficiency contributes to diabetic nephropathy in fat-fed ApoE-/- mice. PLoS One 9:e92952 (2014). Mouse . PubMed: 24667560

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Question

Please provide a concentration for ab30719 lot number 199233

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Answer

Thank you for your enquiry.

I can confirm the concentration of lot 199233 is 6.47mg/ml.

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

We are interested in the anti-creatinine antibody for use in a sandwich assay. I see on the website that Abcam has approved the antibody for competitive ELISA only. Does this mean that other assays were not performed, or that other assays were performed and not successful? I am aware that creatinine is a very small molecule, and we typically work with proteins in sandwich assays. It seems that the nature of the small moleculemay not allow for a sandwich to be formed.
I am writing to ask, again, what it means that certain assays are not approved on the data sheet.

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Answer

Thank you for contacting Abcam.

If we do mention certain applications on the datasheet, that doesn’t mean that it will not work for that application, just that we have not tested in that application (same for species). If we have tested in in an application and we know it will not work, then we will clearly state that on the datasheet.

If you do want to test the antibodies in an untested application, then you can take advantage of our 100% Abreview Testing Discount program:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If there is anything else I can help you with, please let me know.

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Question

send competitive ELISA protocol

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Answer

Thank you for contacting us.

The protocol for the competitive ELISA is as follows:

Protocol:

Coat the wells of an enhanced binding 96 well polystyrene microtiter plate with the Ig fraction of the antiserum, diluted in 10mM Tris, pH8.5 (125ul/well).
Incubate the plate for 2 hours at 37 degrees C.
Wash the plate 4 times with Tris buffered saline containing Tween 20and tap dry.
Prepare standard solutions of the target drug in TBST at 0 and 10ng/ml and add to the appropriate wells (50ul/well).
Add 75ul of conjugate (derivative of target drug conjugated to HRP), diluted in Tris buffer (pH7.2) containing preservatives with stabilising agentsto each of the wells.
Incubate the plate for 1 hour at 25 degrees C.
Wash the plate 6 times over a 10 minute period with TBST.
Add 125ul of tetramethylbenzidine (TMB) substrate solution to each well of the plate.
Incubate for 15 to 20 minutes in the dark at room temperature.
Terminate the reaction by addition of 125ul 0.2M H2SO4 to each well.
Measure absorbance at 450nm using a microtiter plate reader.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

wir moechten einen CreatininAssay etablieren (ohne Enzymreaktion): AK immobilisieren, dann gekoppeltes Ag (Goldpartikel ueber COOH) ueber Affinitaetsbindung messen. Gibt es so etwas schon? Haben Sie Erfahrungen?

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Answer

Vielen Dank für Ihren Anruf.

Wie versprochen haben ich das Labor, das den Kreatininantikörper ab30719 herstellt, kontaktiert, und wir können Ihnen bestätigen, dass der Antikörper sowohl freies (ungebundenes) als auch Carrierprotein-konjugiertes Kreatinin detektieren kann. Von dieser Seite sollte es also kein Problem sein, wenn Sie das Kreatinin konjugieren möchten.

Des Weiteren habe ich bezüglich der Kopplungsreaktion für unsere Easy Link-Kits nachgefragt: In der Tat könnte es theoretisch möglich sein, Kreatinin über die Aminogruppe zu konjugieren. Allerdings liegen keine praktischen Erfahrungen darüber vor, und daher können wir diese Anwendung leider nicht garantieren. Wenn Sie dies für ein Konjugat Ihrer Wahl jedoch selbst testen möchten, würde ich Ihnen einen Kit mit den Kleingroessen 3x10 ug empfehlen (zum Beispiel ab102908). Da die Kits für die Kopplung von Antikörpern und Proteinen optimiert sind, würden wir Ihnen eine Optimierung der einzusetzenden Mengen empfehlen. Gegebenenfalls ist es auch vonnöten, die Proben aufzureinigen (zum Beispiel um BSA oder andere Proteine zu entfernen) oder aufzukonzentrieren.

Da wir auf diesem Gebiet der Kreatinin-Detektion jedoch keine Experten sind, können wir leider keine projektbezogenen Ratschläge geben. Vielleicht bringt eine Recherche in PubMed oder anderen relevanten Quellen Einblicke darüber, welche Art von Assays schon entwickelt wurden und für Ihr Vorhaben von Interesse sein könnten.

Ich möchte mich dafür entschuldigen, dass ich nicht mehr Informationen für Sie habe und hoffe, dass diese Angaben dennoch hilfreich für Sie sind. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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