Creatinine Assay Kit (ab65340)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cerebral Spinal Fluid, Other biological fluids, Serum, Urine
Overview
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Product name
Creatinine Assay Kit
See all Creatinine kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Serum, Other biological fluids, Cerebral Spinal Fluid -
Assay type
Quantitative -
Assay time
1h 00m -
Product overview
Creatinine Assay Kit ab65340 provides an accurate, convenient measure of creatinine concentration in biological fluids such as serum, urine or CSF.
In the creatinine assay protocol, creatinine is converted to creatine by creatininase, creatine is converted to sarcosine, which is specifically oxidized to produce a product which reacts with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 538/587 nm).
Unlike picric acid assays, this kit is suitable for serum creatinine determinations, as well as for urine and other biological samples.
Creatinine assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 60 min at 37ºC
- analyze with microplate reader
For deproteinization of samples: Better results are typically seen with this assay when using a 10kda filter for sample deproteinization than when using the PCA method.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K625 Creatinine Colorimetric/Fluorometric Assay Kit. K625-100 is the same size as the 100 test size of ab65340.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2000 tests Assay Buffer II 1 x 25ml 20 x 25ml Creatinase 1 vial 20 vials Creatininase 1 vial 20 vials Sarcosine Enzyme Mix 1 vial 20 vials Creatinine Standard 1 vial 20 vials OxiRed Probe 1 x 200µl 20 x 200µl -
Research areas
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Relevance
Creatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.
Associated products
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Related Products
Images
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Mice were injected i.p. with 6-ECDCA before I/R injury. At 24 h after I/R, kidney, serum and urine samples were collected for measurements of creatinine in serum with ab65340
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Creatinine measured in mouse and human serum plotted against RFU. Samples were diluted 5-10 fold.
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Creatinine measured in mouse and human urine plotted against RFU. Samples were diluted 400-800 fold.
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Creatinine levels in filtered human urine was measured in the presence or absence of creatininase (background signal subtracted.
Datasheets and documents
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SDS download
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Datasheet download
References (126)
ab65340 has been referenced in 126 publications.
- Gao Q et al. Inhibition of DNA methyltransferase aberrations reinstates antioxidant aging suppressors and ameliorates renal aging. Aging Cell 21:e13526 (2022). PubMed: 34874096
- Vallés-Saiz L et al. Microtubule-associated protein tau in murine kidney: role in podocyte architecture. Cell Mol Life Sci 79:97 (2022). PubMed: 35084555
- Widjaja AA et al. A Neutralizing IL-11 Antibody Improves Renal Function and Increases Lifespan in a Mouse Model of Alport Syndrome. J Am Soc Nephrol 33:718-730 (2022). PubMed: 35140116
- Tsai SC et al. Anti-Obesity Effect of Nostoc commune Ethanol Extract In Vitro and In Vivo. Nutrients 14:N/A (2022). PubMed: 35267941
- Chen HQ et al. Effects of Astragalus membranaceus on systemic lupus erythematosus in a mouse model of pregnancy. Immun Inflamm Dis 10:e624 (2022). PubMed: 35634952