Overview

  • Product name

    Creatinine Assay Kit (Colorimetric)
    See all Creatinine kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    7.981 mg/dl 20 = 2.159%
    7.452 mg/dl 20 = 3.203%
    2.007 mg/dl 20 = 5.995%
    7.326 mg/dl 20 = 3.729%
    Inter-assay
    Sample n Mean SD CV%
    7.591 mg/dl 20 = 2.249%
    7.232 mg/dl 20 = 2.052%
    1.932 mg/dl 20 = 2.027%
    7.085 mg/dl 20 = 1.433%
  • Sample type

    Urine
  • Assay type

    Quantitative
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine = 95 92% - 99%

  • Assay time

    0h 30m
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Abcam's Creatinine Assay Kit (Colorimetric) (ab204537) is a complete kit for the quantitative determination of creatinine in urine, and is based upon the Jaffe reaction.

    Sensitivity = 0.042 mg/dL.

    General range = 0.31 - 20 mg/dL

  • Notes

    Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP. Creatine either comes from the diet or is synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist. Creatine and p-creatine are converted nonenzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo, this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine, and under normal conditions, its formation occurs at a relatively constant rate. Intra-individual variation of creatinine levels is <15% from day to day, making it a useful marker for normalizing levels of other molecules found in urine. Altered creatinine levels may be associated with conditions that result in decreased renal blood flow, such as diabetes and cardiovascular disease.

     

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 2 x 96 tests
    Clear Microtiter Plates 2 units
    Creatinine Detection Reagent 1 x 20ml
    Creatinine Standard 1 vial
    Plate Sealer 2 units
  • Research areas

  • Relevance

    Creatinine, or creatine anhydride, is a breakdown product of creatine phosphate in muscle. The loss of water molecule from creatine results in the formation of creatinine. Creatinine is transferred to the kidneys by blood plasma, whereupon it is eliminated from the body by glomerular filtration and partial tubular excretion. Creatinine is usually produced and excreted at a fairly constant rate, and blood creatinine is used to determine glomerular filtration rate (GFR), a measure of kidney function.

Images

  • Typical standard curve obtained using Creatinine Assay Kit (Colorimetric) (ab204537) which is a complete kit for the quantitative determination of creatinine in urine, and is based upon the Jaffe reaction.

Protocols

References

ab204537 has not yet been referenced specifically in any publications.

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