• Product name
  • Description
    Rabbit polyclonal to CREB
  • Host species
  • Specificity
    Immunogen has 92% alignment to ATF-1
  • Tested applications
    Suitable for: ChIP, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) - non-phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.

  • Positive control
    • HeLa cell extract. Human breast carcinoma. This antibody gave a positive result when used in the following methanol fixed cell lines: A549



Our Abpromise guarantee covers the use of ab31387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 20622856
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa.
ELISA 1/20000.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.


  • Function
    This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
  • Involvement in disease
    Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
  • Sequence similarities
    Belongs to the bZIP family.
    Contains 1 bZIP domain.
    Contains 1 KID (kinase-inducible) domain.
  • Post-translational
    Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Active transcription factor CREB antibody
    • cAMP response element binding protein 1 antibody
    • cAMP response element binding protein antibody
    • cAMP responsive element binding protein 1 antibody
    • cAMP-responsive element-binding protein 1 antibody
    • CREB antibody
    • CREB-1 antibody
    • CREB1 antibody
    • CREB1_HUMAN antibody
    • Cyclic AMP-responsive element-binding protein 1 antibody
    • MGC9284 antibody
    • OTTHUMP00000163864 antibody
    • OTTHUMP00000163865 antibody
    • OTTHUMP00000206660 antibody
    • OTTHUMP00000206662 antibody
    • OTTHUMP00000206667 antibody
    • Transactivator protein antibody
    see all


  • ICC/IF image of ab31387 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31387 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CREB antibody (ab31387) at 1/1000 dilution

    Lane 1 : Mouse primary hippocampal neurons whole cell lysate
    Lane 2 : Rat primary hippocampal neurons whole cell lysate

    Lysates/proteins at 30 µg per lane.

    All lanes : Undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

    Exposure time: 10 seconds

    See Abreview

  • Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 ab31387. Left: Untreated sample; Right: Sample treated with synthesized peptide (negative control).
  • Lane 1 : Anti-CREB antibody (ab31387) at 1/500 dilution
    Lane 2 : Anti-CREB antibody (ab31387) at 1/500 dilution (treated with synthesized peptide )

    All lanes : HeLa cell extract

    Predicted band size: 37 kDa


This product has been referenced in:
  • Kluge M  et al. Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-ß1 in salivary ducts. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30648263) »
  • Zhang F  et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). Read more (PubMed: 30651859) »
See all 19 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Rat Cell (Hippocampal primary Neurons)
Yes - Triton 0.1% PBS
Hippocampal primary Neurons
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 06 2014

Western blot
Rat Cell lysate - whole cell (Primary hippocampal neurons)
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
30 µg
Primary hippocampal neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 14 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Human Cell lysate - whole cell (Ovarian carcinoma)
Negative control
5' UTR of gene of interest (no CREB binding sites)
Ovarian carcinoma
Detection step
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control
CREB binding site in the FOS promoter

Abcam user community

Verified customer

Submitted Jan 21 2012


Thank you for contacting us. Please find the requested MSDS documents attached. Please let me know if you need any additional information.

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Thank you for contacting us. I have asked the lab  what they consider a positive ChIP result. I think they will want to know how you calculated the 0.09 - fold enrichment. You also mentiioned a 1.5 - fold enrichment for a positive control. Can you tell me what that control was? There can be a wide range of values depending on the calculation. For example, the datasheet for our popular histone H3 antibody ab1791 has ChIP data values well below 0.09 but those values are presented as evidence of the antibody's efficacy. On the other hand, at least one customer review of the same antibody has data with values between 1 and 3.  

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Gracias por ponerse en contacto con nosotros. Hemos llevado a cabo un alineamiento de secuencia mediante BLAST, y existe un 92% de coincidencia con ATF-1. Aunque nosotros no lo hemos testado específicamente en el laboratorio, es muy posible que exista reactividad cruzada entre ambas proteínas. Respecto a las referencias solicitadas, siento no poder ofrecerle ninguna otra referencia en cuanto al uso de este anticuerpo en ChIPs. Toda la información disponible se encuentra en la ficha técnica del producto y tan pronto como dispongamos de información adicional, ésta será actualizada en las datasheets. Espero que esta información le sea de utilidad. Por favor, para cualquier otra duda, información o sugerencia, no dude en ponerse en contacto con nosotros.

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