Product nameAnti-CREB antibody
See all CREB primary antibodies
DescriptionRabbit polyclonal to CREB
SpecificityImmunogen has 92% alignment to ATF-1
Tested applicationsSuitable for: ChIP, WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (Human) - non-phosphopeptide derived from human CREB around the phosphorylation site of Serine 133.
- HeLa cell extract. Human breast carcinoma. This antibody gave a positive result when used in the following methanol fixed cell lines: A549
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab31387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 20622856|
|WB||1/500 - 1/1000. Predicted molecular weight: 37 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 µg/ml.|
FunctionThis protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
Involvement in diseaseDefects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
Sequence similaritiesBelongs to the bZIP family.
Contains 1 bZIP domain.
Contains 1 KID (kinase-inducible) domain.
modificationsStimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
- Information by UniProt
- Active transcription factor CREB antibody
- cAMP response element binding protein 1 antibody
- cAMP response element binding protein antibody
ICC/IF image of ab31387 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab31387 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-CREB antibody (ab31387) at 1/1000 dilution
Lane 1 : Mouse primary hippocampal neurons whole cell lysate
Lane 2 : Rat primary hippocampal neurons whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : Undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 10 seconds
Immunohistochemical analysis of paraffin embedded breast carcinoma tissue sections, using 1/50 ab31387. Left: Untreated sample; Right: Sample treated with synthesized peptide (negative control).
Lane 1 : Anti-CREB antibody (ab31387) at 1/500 dilution
Lane 2 : Anti-CREB antibody (ab31387) at 1/500 dilution (treated with synthesized peptide )
All lanes : HeLa cell extract
Predicted band size: 37 kDa
This product has been referenced in:
- Kluge M et al. Differential regulation of vacuolar H+ -ATPase subunits by transforming growth factor-ß1 in salivary ducts. J Cell Physiol N/A:N/A (2019). Read more (PubMed: 30648263) »
- Zhang F et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). Read more (PubMed: 30651859) »