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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper HLH / Leucine Zipper
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Anti-CREB (phospho S121) antibody (ab60941)

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Western blot - Anti-CREB (phospho S121) antibody (ab60941)

    Key features and details

    • Rabbit polyclonal to CREB (phospho S121)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Recombinant Rat CREB protein (ab43602)

    View more associated products

    Overview

    • Product name

      Anti-CREB (phospho S121) antibody
      See all CREB primary antibodies
    • Description

      Rabbit polyclonal to CREB (phospho S121)
    • Host species

      Rabbit
    • Specificity

      Detects endogenous levels of CREB only when phosphorylated at serine 121.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Synthetic phosphopeptide derived from human CREB around the phosphorylation site of serine 121 (T-D-SP-Q-K).

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • HLH / Leucine Zipper
      • HLH / Leucine Zipper
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Immunology
      • Innate Immunity
      • TLR Signaling

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Rat CREB protein (ab43602)

    Applications

    Our Abpromise guarantee covers the use of ab60941 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 37 kDa).

    Target

    • Function

      This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Implicated in synchronization of circadian rhythmicity.
    • Involvement in disease

      Defects in CREB1 may be a cause of angiomatoid fibrous histiocytoma (AFH) [MIM:612160]. A distinct variant of malignant fibrous histiocytoma that typically occurs in children and adolescents and is manifest by nodular subcutaneous growth. Characteristic microscopic features include lobulated sheets of histiocyte-like cells intimately associated with areas of hemorrhage and cystic pseudovascular spaces, as well as a striking cuffing of inflammatory cells, mimicking a lymph node metastasis. Note=A chromosomal aberration involving CREB1 is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(2;22)(q33;q12) with CREB1 generates a EWSR1/CREB1 fusion gene that is most common genetic abnormality in this tumor type.
    • Sequence similarities

      Belongs to the bZIP family.
      Contains 1 bZIP domain.
      Contains 1 KID (kinase-inducible) domain.
    • Post-translational
      modifications

      Stimulated by phosphorylation. Phosphorylation of both Ser-133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
      Sumoylated by SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P16220 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1385 Human
      • Entrez Gene: 12912 Mouse
      • Entrez Gene: 81646 Rat
      • Omim: 123810 Human
      • SwissProt: P16220 Human
      • SwissProt: Q01147 Mouse
      • SwissProt: P15337 Rat
      • Unigene: 516646 Human
      • Unigene: 453295 Mouse
      • Unigene: 90061 Rat
      see all
    • Alternative names

      • Active transcription factor CREB antibody
      • cAMP response element binding protein 1 antibody
      • cAMP response element binding protein antibody
      • cAMP responsive element binding protein 1 antibody
      • cAMP-responsive element-binding protein 1 antibody
      • CREB antibody
      • CREB-1 antibody
      • CREB1 antibody
      • CREB1_HUMAN antibody
      • Cyclic AMP-responsive element-binding protein 1 antibody
      • MGC9284 antibody
      • OTTHUMP00000163864 antibody
      • OTTHUMP00000163865 antibody
      • OTTHUMP00000206660 antibody
      • OTTHUMP00000206662 antibody
      • OTTHUMP00000206667 antibody
      • Transactivator protein antibody
      see all

    Images

    • Western blot - Anti-CREB (phospho S121) antibody (ab60941)
      Western blot - Anti-CREB (phospho S121) antibody (ab60941)
      All lanes : Anti-CREB (phospho S121) antibody (ab60941) at 1/500 dilution

      Lane 1 : HeLa cell extracts treated
      with Etoposide (25uM, 24hours)
      Lane 2 : HeLa cell extracts treated
      with Etoposide (25uM, 24hours) with immunizing peptide

      Predicted band size: 37 kDa
      Observed band size: 42 kDa
      why is the actual band size different from the predicted?

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab60941? Please let us know so that we can cite the reference in this datasheet.

    ab60941 has not yet been referenced specifically in any publications.

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