• Product name
  • Description
    Rabbit polyclonal to CREB3
  • Host species
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide: YSSDTRGSLP AEHGVLSRQL RALPSEDPYQ LELPALQSEV PKDSTHQWLD , corresponding to Internal sequence amino acids 247-296 of Human CREB3



Our Abpromise guarantee covers the use of ab42454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.


  • Relevance
    This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element, an octameric palindrome. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. An additional transcript variant has been identified, but its biological validity has not been determined.
  • Cellular localization
    Isoform 2: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Membrane. Isoform 3: Nucleus. Cytoplasm.
  • Database links
  • Alternative names
    • Basic leucine zipper protein antibody
    • cAMP responsive element binding protein 3 antibody
    • Clic AMP responsive element binding protein 3 antibody
    • CREB 3 antibody
    • cyclic AMP response element (CRE) binding protein/activating transcription factor 1 antibody
    • Cyclic AMP responsive element binding protein 3 antibody
    • Leucine zipper protein antibody
    • LUMAN antibody
    • LZIP antibody
    • MGC15333 antibody
    • MGC19782 antibody
    • Transcription factor LZIP alpha antibody
    see all


  • All lanes : Anti-CREB3 antibody (ab42454) at 0.5 µg/ml

    Lane 1 : MW Markers
    Lane 2 : Jurkat Cell lysate at 10 µg

    All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 41 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.

    Suggested dilution of 0.5µg/ml in 5% skim milk / PBS buffer. Gel concentration of 12%.


This product has been referenced in:
  • Xue H  et al. A Novel Tumor-promoting Mechanism of IL6 and the Therapeutic Efficacy of Tocilizumab: Hypoxia-induced IL6 Is a Potent Autophagy Initiator in Glioblastoma via the p-STAT3-MIR155-3p-CREBRF Pathway. Autophagy N/A:0 (2016). WB ; Human . Read more (PubMed: 27163161) »
  • Reiling JH  et al. A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens. Nat Cell Biol 15:1473-85 (2013). Read more (PubMed: 24185178) »
See all 2 Publications for this product

Customer reviews and Q&As


I don't expect any problems with the ab4570 as this one works fine in IP as well, however, I am not sure about the ab42454.

Nonetheless, I am not too concerned either: ab42454 is not tested in IP, but it works in ELISA, which is a good indicator that it will react with the native protein.

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