Overview

  • Product name

    Anti-CREB3 antibody [EPR14215(B)]
    See all CREB3 primary antibodies
  • Description

    Rabbit monoclonal [EPR14215(B)] to CREB3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CREB3 aa 50-150. The exact sequence is proprietary.
    Database link: O43889

  • Positive control

    • HeLa and 293 cells; HeLa, HepG2 and 293 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 44 kDa).

Target

  • Relevance

    This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds to the cAMP-responsive element, an octameric palindrome. The protein interacts with host cell factor C1, which also associates with the herpes simplex virus (HSV) protein VP16 that induces transcription of HSV immediate-early genes. This protein and VP16 both bind to the same site on host cell factor C1. It is thought that the interaction between this protein and host cell factor C1 plays a role in the establishment of latency during HSV infection. An additional transcript variant has been identified, but its biological validity has not been determined.
  • Cellular localization

    Isoform 2: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Membrane. Isoform 3: Nucleus. Cytoplasm.
  • Database links

  • Alternative names

    • Basic leucine zipper protein antibody
    • cAMP responsive element binding protein 3 antibody
    • Clic AMP responsive element binding protein 3 antibody
    • CREB 3 antibody
    • cyclic AMP response element (CRE) binding protein/activating transcription factor 1 antibody
    • Cyclic AMP responsive element binding protein 3 antibody
    • Leucine zipper protein antibody
    • LUMAN antibody
    • LZIP antibody
    • MGC15333 antibody
    • MGC19782 antibody
    • Transcription factor LZIP alpha antibody
    see all

Images

  • Anti-CREB3 antibody [EPR14215(B)] (ab180119) at 1/2000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-CREB3 antibody [EPR14215(B)] (ab180119) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 cells labeling CREB3 with ab180119 at 1/100 dilution. Counterstained with DAPI (blue).

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling CREB3 with ab180119 at 1/100 dilution (red) compared to a rabbit IgG control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/75 dilution.

References

ab180119 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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