Product nameAnti-CREB3L1 / OASIS antibody
See all CREB3L1 / OASIS primary antibodies
DescriptionRabbit polyclonal to CREB3L1 / OASIS
Specificityab33051 recognises CREB3L1 / OASIS.
Tested applicationsSuitable for: ELISA, WB, IHC (PFA fixed), IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
- Fetal small intestine lysate.
Previously labelled as CREB3L1.
Previously labelled as CREB3L1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 57 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC (PFA fixed)||Use at an assay dependent concentration. PubMed: 25359725|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionTranscription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. Specifically involved in ER-stress response in astrocytes in the central nervous system (By similartity). May play a role in gliosis. In vitro, binds to box-B element, cAMP response element (CRE) and CRE-like sequences, and activates transcription through box-B element but not through CRE.
Tissue specificityUbiquitously expressed with high levels in pancreas and prostate. Expressed at relatively lower levels in brain.
Sequence similaritiesBelongs to the bZIP family. ATF subfamily.
Contains 1 bZIP domain.
modificationsControlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage is performed sequentially by site-1 and site-2 proteases (PS1 and PS2) and is triggered by translocation to the Golgi apparatus.
Cellular localizationEndoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
- Information by UniProt
- BBF-2 homolog antibody
- CAMP responsive element binding protein 3 like 1 antibody
- cAMP-responsive element-binding protein 3-like protein 1 antibody
Anti-CREB3L1 / OASIS antibody (ab33051) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + fetal small intestine lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 57 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Gel concetration: 12%
ab33051 staining CREB3L1 / OASIS in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked using 1.5% serum for 1 hour at 25°C and then incubated with ab33051 at a 1/800 dilution for 18 hours at 4°C. The secondary used was an undiluted HRP conjugated polyclonal.
Immunofluorescence analysis of U2-OS, staining CREB3L1 / OASIS with ab33051.
Cells were fixed with formaldehyde, permeabilized with 0.5% NP-40 in PBS and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA in PBS) for 12 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.
This product has been referenced in:
- Keller RB et al. Monoallelic and biallelic CREB3L1 variant causes mild and severe osteogenesis imperfecta, respectively. Genet Med 20:411-419 (2018). WB . Read more (PubMed: 28817112) »
- Zhu HY et al. Up-regulation of FGFBP1 signaling contributes to miR-146a-induced angiogenesis in human umbilical vein endothelial cells. Sci Rep 6:25272 (2016). WB . Read more (PubMed: 27121396) »