Transcriptional activator which is processed and activated during endoplasmic reticulum stress late phase. Binds to the cAMP response element (CRE) and activates transcription through CRE. Regulates the transcription of unfolded protein response target genes, preventing accumulation of unfolded proteins in damaged neurons. Also regulates the expression of SEC23A, accelerating protein trafficking from the ER to the Golgi thereby playing a key role in chondrocyte differentiation and formation of epiphyseal cartilage.
Widely expressed with highest levels in placenta, lung, spleen and intestine, and lowest levels in heart, brain, skeletal muscle, thymus, colon and leukocytes. In fetal tissues, the weakest expression is detected in brain and heart.
Involvement in disease
A chromosomal aberration involving CREB3L2 is found in low grade fibromyxoid sarcoma (LGFMS). Translocation t(7;16)(q33;p11) with FUS.
Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP (basic-leucine zipper) domain.
Controlled by regulated intramembrane proteolysis (RIP). Following ER stress a fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases (PS1 and PS2).
Endoplasmic reticulum membrane and Nucleus. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus.
cAMP responsive element binding protein 3 like 2 antibody
cAMP-responsive element-binding protein 3-like protein 2 antibody
Cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
FUS/BBF2H7 protein antibody
Processed cyclic AMP-responsive element-binding protein 3-like protein 2 antibody
Western blot - Anti-CREB3L2 antibody (ab102989)
Anti-CREB3L2 antibody (ab102989) at 1/100 dilution + HeLa cell lysate at 35 µg
Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREB3L2 antibody (ab102989)Image courtesy of Leilani Beltran by Abreview.
ab102989 staining CREB3L2 in murine liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed. Samples were then blocked with 1.5% serum for 1 hour at 25°C and then incubated with ab102989 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an undiluted biotin conjugated goat polyclonal.
Immunocytochemistry/ Immunofluorescence - Anti-CREB3L2 antibody (ab102989)This image is courtesy of an anonymous Abreview
Immunofluorescence analysis of U2-OS, staining CREB3L2 with ab102989.
Cells were fixed with formaldehyde, permeabilized with 0.5% NP-40 in PBS and blocked with 3% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 3% BSA in PBS) for 12 hours at 4°C. An AlexaFluor®546-conjugated goat anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.