Product nameAnti-CREBBP antibody
See all CREBBP primary antibodies
DescriptionRabbit polyclonal to CREBBP
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
Recombinant fragment corresponding to Human CREBBP aa 2123-2442 (C terminal).
Database link: Q92793
- WB: HeLa IHC-P: mouse fore brain, human breast carcinoma, mouse muscle. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab137334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.
Antigen Retrieval: EDTA based, pH 8.0 buffer, 15min
|WB||1/500 - 1/3000. Predicted molecular weight: 265 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionAcetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
Involvement in diseaseNote=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
Sequence similaritiesContains 1 bromo domain.
Contains 1 KIX domain.
Contains 2 TAZ-type zinc fingers.
Contains 1 ZZ-type zinc finger.
DomainThe KIX domain mediates binding to HIV-1 Tat.
modificationsMethylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
Cellular localizationCytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
- Information by UniProt
- CBP antibody
- CBP_HUMAN antibody
- CREB binding protein antibody
All lanes :
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CREBBP knockout whole cell lysate
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 265 kDa
Immunofluorescence staining of paraformaldehyde-fixed (4% paraformaldehyde, 15 minutes at RT) HeLa cells stained for CREBBP with ab137334 at 1:1000 dilution. Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Immunohistochemical analysis of paraffin embedded mouse fore brain tissue labelling CREBBP with ab137334 antibody at 1/750 dilution.
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue labelling CREBBP with ab137334 antibody at 1/750 dilution.
Immunohistochemical analysis of paraffin embedded mouse muscle tissue labelling CREBBP with ab137334 antibody at 1/750 dilution.
Anti-CREBBP antibody (ab137334) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 265 kDa
ab137334 has been referenced in 1 publication.
- Yang F et al. Sulforaphane induces autophagy by inhibition of HDAC6-mediated PTEN activation in triple negative breast cancer cells. Life Sci 213:149-157 (2018). PubMed: 30352240