Overview

  • Product name

  • Description

    Rabbit polyclonal to CREBBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Mouse brain tissue lysate. MOLT4, PC-3 and A549 whole cell lysate.

Applications

Our Abpromise guarantee covers the use of ab235270 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 265 kDa.

Target

  • Function

    Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
  • Involvement in disease

    Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
    Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
  • Sequence similarities

    Contains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • Domain

    The KIX domain mediates binding to HIV-1 Tat.
  • Post-translational
    modifications

    Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
  • Cellular localization

    Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBP antibody
    • CBP_HUMAN antibody
    • CREB binding protein antibody
    • CREB-binding protein antibody
    • Crebbp antibody
    • Cyclic AMP responsive enhancer binding protein antibody
    • KAT3A antibody
    • RSTS antibody
    • RTS antibody
    • Rubinstein Taybi syndrome antibody
    see all

Images

  • All lanes : Anti-CREBBP antibody (ab235270) at 5 µg/ml

    Lane 1 : Mouse brain whole tissue lysate
    Lane 2 : Human eye tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG [H&L] HRP at 1/70000 dilution

    Predicted band size: 265 kDa


    Exposure time: 60 seconds


    7.5% gel.

  • All lanes : Anti-CREBBP antibody (ab235270) at 5 µg/ml

    Lane 1 : MOLT-4 (Human lymphoblastic luekemia cell line) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 5 : Mouse brain whole tissue lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit [H&L] HRP at 1/70000 dilution

    Predicted band size: 265 kDa


    Exposure time: 30 seconds

References

ab235270 has not yet been referenced specifically in any publications.

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