Anti-CREBBP antibody - ChIP Grade (ab10490)

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Overview

  • Product name

    Anti-CREBBP antibody - ChIP Grade
    See all CREBBP primary antibodies

  • Description

    Rabbit polyclonal to CREBBP - ChIP Grade

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ChIP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human CREBBP aa 2392-2442.
    Database link: Q92793
    (Peptide available as ab4916)

  • General notes


    Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.

Properties

Applications

Our Abpromise guarantee covers the use of ab10490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 1-4 µg/mg of lysate.
ChIP Use 2-4µg for 106 cells.
WB 1/1000 - 1/25000. Predicted molecular weight: 265 kDa.Can be blocked with Human CREBBP peptide (ab4916).
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.

  • Involvement in disease

    Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
    Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.

  • Sequence similarities

    Contains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.

  • Domain

    The KIX domain mediates binding to HIV-1 Tat.

  • Post-translational
    modifications

    Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.

  • Cellular localization

    Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CBP antibody
      • CBP_HUMAN antibody
      • CREB binding protein antibody
      • CREB-binding protein antibody
      • Crebbp antibody
      • Cyclic AMP responsive enhancer binding protein antibody
      • KAT3A antibody
      • RSTS antibody
      • RTS antibody
      • Rubinstein Taybi syndrome antibody
      see all

    Images

    • ChIP - Anti-CREBBP antibody - ChIP Grade (ab10490)
      ChIP - Anti-CREBBP antibody - ChIP Grade (ab10490)

      Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab10490 to CREBBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive), values are % of inputs.  2–4 µg of ab10490 and 1-2x106 cells were used in each ChIP experiment.

      Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody - ChIP Grade (ab10490)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody - ChIP Grade (ab10490)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CREBBP with ab10490 at 1/500 (2µg/ml). Detection: DAB.

    • Immunoprecipitation - Anti-CREBBP antibody - ChIP Grade (ab10490)
      Immunoprecipitation - Anti-CREBBP antibody - ChIP Grade (ab10490)

      ab10490 immunoprecipitation of CREBBP.

      Samples: Nuclear (NE) or cytoplasmic (S100) extract (10 mg) from HeLa cells. Antibody: ab10490 used at 20 µg/10 mg extract. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is CREB Binding Protein.

    References

    ab10490 has not yet been referenced specifically in any publications.

    Publishing research using ab10490? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    I am looking for a CBP or P300 antibody to use ChIP assay using zebrafish embryos. What would you recommend from your antibody list? Please let me know if you find any. Thank you so much in advance.

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    Answer

    Thank you for contacting us. We have the following antibodies in our catalog that where tested in ChIP: for CBP: ab2832, ab10489, and ab10490 for p300: ab14984 None of the antibodies has been tested so far in zebrafish. If you could send me please the protein sequences for zebrafish, then I can check the homology. If the homology is about 85% or higher, you can participate in our testing discount program. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. Please let me know the zebrafish sequence and I can check which of the antibodies might be suitable to try in this species. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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