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    crebbp-antibody-chip-grade-ab10490.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-CREBBP antibody - ChIP Grade (ab10490)

  • Datasheet
  • SDS
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ChIP - Anti-CREBBP antibody - ChIP Grade (ab10490)
  • Western blot - Anti-CREBBP antibody - ChIP Grade (ab10490)
  • Immunoprecipitation - Anti-CREBBP antibody - ChIP Grade (ab10490)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody - ChIP Grade (ab10490)

Key features and details

  • Rabbit polyclonal to CREBBP - ChIP Grade
  • Suitable for: IP, ChIP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Primary
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Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)
Protein
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Recombinant human CREBBP protein (ab167964)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CREBBP antibody - ChIP Grade
    See all CREBBP primary antibodies
  • Description

    Rabbit polyclonal to CREBBP - ChIP Grade
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ChIP
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human CREBBP aa 2392-2442.
    Database link: Q92793
    (Peptide available as ab4916)

  • Positive control

    • WB: HeLa whole cell lysate
  • General notes


    Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Antibodies were affinity purified using the peptide immobilized on solid support.
  • Primary antibody notes

    Cyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Epigenetics and Nuclear Signaling
    • Bromodomains

Associated products

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human CREBBP protein (ab167964)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab10490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ChIP
Human
IHC-P
Human
IP
Human
WB
Human
All applications
Mouse
Chimpanzee
Rhesus monkey
Gorilla
Orangutan
Application Abreviews Notes
IP
Use at 1-4 µg/mg of lysate.
ChIP
Use 2-4µg for 106 cells.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
IP
Use at 1-4 µg/mg of lysate.
ChIP
Use 2-4µg for 106 cells.
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
  • Involvement in disease

    Note=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
    Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies.
  • Sequence similarities

    Contains 1 bromo domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • Domain

    The KIX domain mediates binding to HIV-1 Tat.
  • Post-translational
    modifications

    Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.
  • Cellular localization

    Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
  • Target information above from: UniProt accession Q92793 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1387 Human
    • Entrez Gene: 12914 Mouse
    • Omim: 600140 Human
    • SwissProt: Q92793 Human
    • SwissProt: P45481 Mouse
    • Unigene: 459759 Human
    • Unigene: 132238 Mouse
    • Unigene: 392384 Mouse
    • Alternative names

      • CBP antibody
      • CBP_HUMAN antibody
      • CREB binding protein antibody
      • CREB-binding protein antibody
      • Crebbp antibody
      • Cyclic AMP responsive enhancer binding protein antibody
      • KAT3A antibody
      • RSTS antibody
      • RTS antibody
      • Rubinstein Taybi syndrome antibody
      see all

    Images

    • ChIP - Anti-CREBBP antibody - ChIP Grade (ab10490)
      ChIP - Anti-CREBBP antibody - ChIP Grade (ab10490)

      Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab10490 to CREBBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive), values are % of inputs.  2–4 µg of ab10490 and 1-2x106 cells were used in each ChIP experiment.

      Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL

       

    • Western blot - Anti-CREBBP antibody - ChIP Grade (ab10490)
      Western blot - Anti-CREBBP antibody - ChIP Grade (ab10490)
      All lanes : Anti-CREBBP antibody - ChIP Grade (ab10490) at 0.4 µg/ml

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg

      Exposure time: 3 minutes


      Detected by chemiluminescence.

    • Immunoprecipitation - Anti-CREBBP antibody - ChIP Grade (ab10490)
      Immunoprecipitation - Anti-CREBBP antibody - ChIP Grade (ab10490)

      ab10490 immunoprecipitating CREBBP at 6 µg/ml lysate.

      Lane 1: Anti-CREBBP antibody ab10489 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).

      Lane 2: Anti-CREBBP antibody ab10490 in HeLa whole cell extract (1 mg per IP reaction; 20% of IP loaded).

      Lane 3: IgG control.

      For blotting immunoprecipitated CREBBP, ab10490 was used at 1 µg/mL.

      Detection: Chemiluminescence with an exposure time of 30 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody - ChIP Grade (ab10490)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CREBBP antibody - ChIP Grade (ab10490)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling CREBBP with ab10490 at 1/500 (2µg/ml). Detection: DAB.

    Protocols

    • ChIP protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab10490? Please let us know so that we can cite the reference in this datasheet.

    ab10490 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I am looking for a CBP or P300 antibody to use ChIP assay using zebrafish embryos. What would you recommend from your antibody list? Please let me know if you find any. Thank you so much in advance.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 08 2011

    Answer

    Thank you for contacting us. We have the following antibodies in our catalog that where tested in ChIP: for CBP: ab2832, ab10489, and ab10490 for p300: ab14984 None of the antibodies has been tested so far in zebrafish. If you could send me please the protein sequences for zebrafish, then I can check the homology. If the homology is about 85% or higher, you can participate in our testing discount program. For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works: The testing discount program is for customers who like to use an antibody/protein on an untested species/application. You would purchase the antibody at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount for ONE antibody at the full price (100%) of the antibody you have tested. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. Please let me know the zebrafish sequence and I can check which of the antibodies might be suitable to try in this species. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Sep 08 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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