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Anti-CRELD1 antibody (ab131286)

  • Datasheet
  • SDS
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Western blot - Anti-CRELD1 antibody (ab131286)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRELD1 antibody (ab131286)

Key features and details

  • Rabbit polyclonal to CRELD1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CRELD1 antibody
  • Description

    Rabbit polyclonal to CRELD1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human CRELD1 aa 100-200 conjugated to keyhole limpet haemocyanin.
    Database link: Q96HD1

  • Positive control

    • This antibody gave a positive result in IHC in the following FFPE tissue: Human heart muscle. This antibody also gave a positive signal within WB in the following tissue lysates: Human Fetal Heart; Human Heart; Mouse Heart; Rat Heart.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Myocardial regeneration

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131286 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P
Use a concentration of 5 µg/ml.

Target

  • Tissue specificity

    Highly expressed in fetal lung, liver, kidney, adult heart, brain and skeletal muscle. Weakly expressed in placenta, fetal brain, and adult lung, liver, kidney and pancreas.
  • Involvement in disease

    Defects in CRELD1 may be the cause of susceptibility to atrioventricular septal defect type 2 (AVSD2) [MIM:606217]. AVSD is a spectrum of cardiac malformations that result in a persistent common atrioventricular canal. The complete form of AVSD involves underdevelopment of the lower part of the atrial septum and the upper part of the ventricular septum. A less severe form, known as partial AVSD or ostium primum atrial septal defect has a deficiency of the atrial septum. Complete AVSD are clinically apparent at birth, whereas less severe forms, such as an isolated cleft mitral valve or small defects of the atrial or ventricular septa may go undetected.
  • Sequence similarities

    Belongs to the CRELD family.
    Contains 2 EGF-like domains.
    Contains 2 FU (furin-like) repeats.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q96HD1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 78987 Human
    • Entrez Gene: 171508 Mouse
    • Entrez Gene: 312638 Rat
    • Omim: 607170 Human
    • SwissProt: Q96HD1 Human
    • SwissProt: Q91XD7 Mouse
    • SwissProt: Q4V7F2 Rat
    • Unigene: 9383 Human
    • Unigene: 41593 Mouse
    • Unigene: 6461 Rat
    see all
  • Alternative names

    • Atrioventricular septal defect 2 antibody
    • AVSD2 antibody
    • CIRRIN antibody
    • CREL1_HUMAN antibody
    • CRELD1 antibody
    • Cysteine rich with EGF like domains 1 antibody
    • Cysteine-rich with EGF-like domain protein 1 antibody
    • DKFZP566D213 antibody
    see all

Images

  • Western blot - Anti-CRELD1 antibody (ab131286)
    Western blot - Anti-CRELD1 antibody (ab131286)
    All lanes : Anti-CRELD1 antibody (ab131286) at 1 µg/ml

    Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
    Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa
    Additional bands at: 70 kDa (possible non-specific binding)


    Exposure time: 4 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab131286 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRELD1 antibody (ab131286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRELD1 antibody (ab131286)

    IHC image of CRELD1 staining in Human heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131286, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab131286? Please let us know so that we can cite the reference in this datasheet.

ab131286 has not yet been referenced specifically in any publications.

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