Overview

  • Product name

    Anti-CRES antibody
  • Description

    Goat polyclonal to CRES
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human CRES aa 23-34 (internal sequence) (Cysteine residue). (NP_005483.1).
    Sequence:

    C-DPKKNETGVLRK


    Database link: O60676

  • Positive control

    • WB: HEK-293 lysate overexpressing human CRES with C-terminal MYC tag.
  • General notes

     This product was previously labelled as CST8

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab223643 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab223643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 16 kDa.

Target

  • Function

    Performs a specialized role during sperm development and maturation.
  • Tissue specificity

    Proximal caput region of the epididymis. Lower expression in the testis. Within the testis it is localized to the elongating spermatids, whereas within the epididymis it is exclusively synthesized by the proximal caput epithelium.
  • Sequence similarities

    Belongs to the cystatin family.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CRES antibody
    • CST 8 antibody
    • Cst8 antibody
    • CST8_HUMAN antibody
    • cystatin 8 (cystatin related epididymal specific) antibody
    • Cystatin 8 antibody
    • Cystatin related epididymal specific antibody
    • Cystatin related epididymal specific protein antibody
    • Cystatin related epididymal spermatogenic protein antibody
    • Cystatin related protein epididymis specific antibody
    • Cystatin-8 antibody
    • Cystatin-related epididymal spermatogenic protein antibody
    see all

Images

  • Lane 1 : Anti-CRES antibody (ab223643) at 0.3 µg/ml
    Lane 2 : Anti-CRES antibody (ab223643) at 1 mg/ml
    Lane 3 : Anti-MYC tag at 1/1000 dilution

    Lanes 1 & 3 : HEK-293 (human epithelial cell line from embryonic kidney) lysate overexpressing human CRES with C-terminal MYC tag (in RIPA buffer)
    Lane 2 : Mock transfected HEK-293 (human epithelial cell line from embryonic kidney) lysate (in RIPA buffer)

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 16 kDa
    Observed band size: 23 kDa
    why is the actual band size different from the predicted?



    Primary incubations were for 1 hour.

References

ab223643 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab223643.
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