Key features and details
- Rabbit polyclonal to CREST
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CREST antibody
See all CREST primary antibodies
DescriptionRabbit polyclonal to CREST
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human CREST aa 47-396.
Database link: O75177
- WB: Mouse kidney, lung and spleen tissue lysates. IHC-P: Human gastric cancer and brain tissues.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab222835 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 30,34,41,43,44 kDa).|
FunctionTranscriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.
Tissue specificityUbiquitous; with lowest levels in spleen.
Sequence similaritiesBelongs to the SS18 family.
DomainThe MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Localizes to nuclear bodies. Co-localizes with SGOL1 at kinetochore.
- Information by UniProt
- Calcium-responsive transactivator antibody
- CREST antibody
- CREST_HUMAN antibody
Paraffin-embedded human gastric cancer tissue stained for CREST using ab222835 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human brain tissue stained for CREST using ab222835 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-CREST antibody (ab222835) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse spleen tissue lysate
All lanes : Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 30,34,41,43,44 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
ab222835 has not yet been referenced specifically in any publications.