Overview

  • Product name

  • Description

    Rabbit polyclonal to CREST
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human CREST (internal sequence). The exact sequence is proprietary.
    Database link: O75177

  • Positive control

    • WB: Mouse brain lysate; HeLa whole cell and nuclear lysates. IP: MCF7 whole cell extract. IHC-P: Huh-7 xenograft tissue. ICC/IF: A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 43 kDa.
IP 1/100 - 1/500.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP.
  • Tissue specificity

    Ubiquitous; with lowest levels in spleen.
  • Sequence similarities

    Belongs to the SS18 family.
  • Domain

    The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Localizes to nuclear bodies. Co-localizes with SGOL1 at kinetochore.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcium-responsive transactivator antibody
    • CREST antibody
    • CREST_HUMAN antibody
    • KIAA0693 antibody
    • LP2261 antibody
    • MGC26711 antibody
    • MGC78386 antibody
    • sarcoma translocation gene on chromosome18like1 antibody
    • SS18 antibody
    • SS18-like protein 1 antibody
    • SS18L1 antibody
    • SS18L1 nBAF chromatin remodeling complex antibody
    • SSXT antibody
    • synovial sarcoma translocation antibody
    • synovial sarcoma translocation gene on chromosome 18-like 1 antibody
    • SYT homolog 1 antibody
    see all

Images

  • Anti-CREST antibody (ab227535) at 1/1000 dilution + Mouse brain lysate at 50 µg

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • All lanes : Anti-CREST antibody (ab227535) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HeLa nuclear lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 43 kDa



    10% SDS-PAGE gel.

  • Paraffin-embedded Huh-7 xenograft tissue stained for CREST with ab227535 at 1/500 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for CREST (green) using ab227535 at 1/500 dilution in ICC/IF.

    Blue: Hoechst 33342 staining.

  • CREST was immunoprecipitated from MCF7 (human breast adenocarcinoma cell line) whole cell extract with 5 μg ab227535. Western blot was performed from the immunoprecipitate using ab227535.

    Lane 1: Control IgG IP in MCF7 whole cell extract.

    Lane 2: ab227535 IP in MCF7 whole cell extract.

References

ab227535 has not yet been referenced specifically in any publications.

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