Overview

  • Product name

  • Description

    Rabbit polyclonal to CRF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide aa 148-196 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P06850

  • Positive control

    • IHC-P: Rat kidney tissue.
  • General notes

     This product was previously labelled as Corticotropin Releasing Factor , Corticotropin Releasing Factor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab216599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.

For IF please use a dilution of 1/50 - 1/200.

Target

  • Relevance

    CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

  • Alternative names

    • Corticoliberin antibody
    • Corticotropin releasing factor antibody
    • Corticotropin releasing hormone antibody
    • CRF antibody
    • Crh antibody
    • CRH1 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of rat kidney tissue labeling CRF with ab216599 at a 1/200 dilution, followed by conjugation with a goat anti-rabbit IgG PE conjugated secondary at 1/200 for 40 minutes at 37°C. DAPI (5 µg/mL, blue) was used to stain the cell nuclei.

  • Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of rat kidney tissue labeling CRF with ab216599 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.

References

ab216599 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Placenta
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Mar 27 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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