Overview

  • Product name

  • Description

    Rabbit polyclonal to CRF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: RIA, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Rat CRF aa 1-41. The exact sequence is proprietary.
    Database link: P01143

  • Positive control

    • IHC-P: Rat brain tissue; Human placenta tissue.
  • General notes

     This product was previously labelled as Corticotropin Releasing Factor , Corticotropin Releasing Factor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab8901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA 1/50000.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

  • Alternative names

    • Corticoliberin antibody
    • Corticotropin releasing factor antibody
    • Corticotropin releasing hormone antibody
    • CRF antibody
    • Crh antibody
    • CRH1 antibody
    see all

Images

  • Ab8901 staining CRF in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000 in blocking buffer) for 16 hours at 21°C. A diluted Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of rat brain tissue, staining CRF with ab8901.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. The figure on the left is a secondary only control.

    See Abreview

  • Radioimmunoassay using ab8901
    - Day 1: Add Recombinant Protein A/G (10ug/ml) to a 96 wells plate; parafilm wrap; incubate overnight at 4°C.
    - Day 2: Aspirate and wash the plate to remove loosely bound Protein A/G; add antibody solution (dilution 1:50000); parafilm wrap; incubate 24h at 4°C.
    - Day 3: Aspirate and wash the plate to remove loosely bound antibody; add CRF standard (0, 0.03, 0.1, 0.3, 1, 3, 10, 30 ng/ml); parafilm wrap; incubate 24h at 4°C.
    - Day 4: DO NOT ASPIRATE; add 125I-CRF; incubate 24h at 4°C.
    - Day 5: Aspirate and wash the plate; break the plate and collect the wells in scintillation tubes; add 2ml of scintillation liquid; count in beta counter (open the 3H window to at least 30-40 keV), at least 2min/tube.

References

This product has been referenced in:

  • Wang Y  et al. Effects of Shugan Hewei Granule on Depressive Behavior and Protein Expression Related to Visceral Sensitivity in a Rat Model of Nonerosive Reflux Disease. Evid Based Complement Alternat Med 2019:1505693 (2019). Read more (PubMed: 30719054) »
  • Tao X  et al. In vitro examination of microglia-neuron crosstalk with BV2 cells, and primary cultures of glia and hypothalamic neurons. Heliyon 4:e00730 (2018). Read more (PubMed: 30148218) »
See all 12 Publications for this product

Customer reviews and Q&As

11-14 of 14 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Feb 17 2009

Answer

ab8901 is provided as whole antiserum - the concentration has not been determined.

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Question
Answer

Yes, this antibody is suitable for use on acetone fixed material

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Answer

This antibody has never been tested in porcine specimins, therefore we can't guarantee results. We currently do not stock any anti-CRF antibodies the are known to cross-react with pig.

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11-14 of 14 Abreviews or Q&A

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