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Can you confirm the correct dilution to use this antibody in RIA? What is the recommended protocol?
Asked on Jan 30 2012
Thank you for contacting Abcam regarding ab8901.
I have confirmed with the laboratory that RIA was not tested in house, but based on the following references:
1. Widmaier EP, Lim AT, Vale W. (1989) Secretion of corticotropin-releasing factor from cultured rat hypothalamic cells: effects of catecholamines. Endocrinol 124:583-590.
2. Vale W, Vaughan J, Yamamoto G, Bruhn T, Douglas C, Dalton D, Rivier C, Rivier J. (1983) Assay of corticotopin releasing factor. Meth Enzymol 103:565-577.
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Answered on Jan 30 2012