Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CRF antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.01% Triton-X
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
The figure on the left is a secondary only control. Red indicates CRF and blue indicates DAPI staining.

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Submitted Jan 04 2013

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