Western blot abreview for Anti-Corticotropin Releasing Factor antibody

Good
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Tissue lysate - whole (Hypothalamus)
Specification
Hypothalamus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2500

Secondary antibody

Name
Non-Abcam antibody was used: Peroxidase-conjugated affinipure Donkey Anti-Rabbi
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Bands
Specific: 25 kDa Non-specific: 17, 23, 35 kDa
Exposure
1 minute(s) and 0 second(s)

Mr. Jean-Christophe Helbling

Verified customer

Submitted Sep 25 2013

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