Recombinant Anti-CRF antibody [EPR19154] (ab184238)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19154] to CRF
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CRF antibody [EPR19154]
See all CRF primary antibodies -
Description
Rabbit monoclonal [EPR19154] to CRF -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human CRF fragment recombinant protein; Human brain, mouse brain, mouse hypothalamus, rat brain and rat hypothalamus lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19154 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 21 kDa). |
Target
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Relevance
CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few). -
Cellular localization
Cytoplasmic and Secreted -
Database links
- Entrez Gene: 1392 Human
- Entrez Gene: 12918 Mouse
- Entrez Gene: 81648 Rat
- Omim: 122560 Human
- SwissProt: P06850 Human
- SwissProt: Q8CIT0 Mouse
- SwissProt: P01143 Rat
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Alternative names
- Corticoliberin antibody
- Corticotropin releasing factor antibody
- Corticotropin releasing hormone antibody
see all
Images
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Anti-CRF antibody [EPR19154] (ab184238) at 1/1000 dilution + Human CRF fragment recombinant protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Human CRF fragment recombinant protein contains aa20-185 with a His-Tag®. This recombinant human fragment protein was made in-house.
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All lanes : Anti-CRF antibody [EPR19154] (ab184238) at 1/5000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse hypothalamus tissue lysate
Lane 4 : Rat brain tissue lysate
Lane 5 : Rat hypothalamus tissue lysate
Lane 6 : Mouse pancreas tissue lysate
Lane 7 : Mouse skin tissue lysate
Lane 8 : Rat skin tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab184238 has been referenced in 4 publications.
- Kavalakatt S et al. Urocortin Neuropeptide Levels Are Impaired in the PBMCs of Overweight Children. Nutrients 14:N/A (2022). PubMed: 35276788
- Di T et al. Dopaminergic afferents from midbrain to dorsolateral bed nucleus of stria terminalis inhibit release and expression of corticotropin-releasing hormone in paraventricular nucleus. J Neurochem 154:218-234 (2020). PubMed: 32096869
- Bo TB et al. Coprophagy prevention alters microbiome, metabolism, neurochemistry, and cognitive behavior in a small mammal. ISME J 14:2625-2645 (2020). PubMed: 32632263
- Tang Y et al. miR-212 mediates counter-regulation on CRH expression and HPA axis activity in male mice. J Mol Endocrinol 59:365-375 (2017). PubMed: 28912247