Overview

  • Product name
  • Description
    Goat polyclonal to CRF1
  • Host species
    Goat
  • Tested applications
    Suitable for: IHC-P, ELISA, IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Sheep, Chicken, Human, Pufferfish, Rhesus monkey, African green monkey, Syrian hamster
  • Immunogen

    Synthetic peptide corresponding to Human CRF1 aa 279-292 (internal sequence) (Cysteine residue). Synthetic peptide:

    KLYYDNEKCWFGKR

    by a Cysteine residue linker, corresponding to internal sequence amino acids 279-292 of Human CRF1(Peptide available as ab113546.)
    Sequence:

    KLYYDNEKCWFGKR


    (Peptide available as ab113546)

Properties

Applications

Our Abpromise guarantee covers the use of ab59023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
ELISA 1/16000. (Peptide)
IHC-FoFr Use at an assay dependent dilution. PubMed: 20383137
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Human CRF1 peptide (ab113546).

Target

  • Function
    This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
  • Tissue specificity
    Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family.
  • Post-translational
    modifications
    C-terminal Ser or Thr residues may be phosphorylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Corticotropin releasing factor type 1 receptor antibody
    • corticotropin releasing hormone receptor 1 antibody
    • Corticotropin-releasing factor receptor 1 antibody
    • Corticotropin-releasing hormone receptor 1 antibody
    • CRF R antibody
    • crf receptor 1 antibody
    • crf receptor type 1 antibody
    • crf type 1 antibody
    • CRF-R antibody
    • CRF-R-1 antibody
    • CRF-R1 antibody
    • CRF1 antibody
    • CRFR antibody
    • CRFR-1 antibody
    • CRFR1 antibody
    • CRFR1_HUMAN antibody
    • CRH-R-1 antibody
    • CRH-R1 antibody
    • CRH-R1h antibody
    • CRHR antibody
    • Crhr1 antibody
    • CRHR1f antibody
    • CRHR1L antibody
    see all

Images

  • ab59023 (1/100) staining CRF1 in paraffin embedded mouse brain tissue sections. Tissues were fixed in paraformaldehyde and permeabilised with 0.3% Triton X100/TBS. Please refer to abreview for further experimental details.

    See Abreview

  • All lanes : Anti-CRF1 antibody (ab59023) at 1 µg/ml

    Lane 1 : Rat Forebrain
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Cerebellum Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa
    Additional bands at: 110 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes

References

This product has been referenced in:
  • Feng Y  et al. Human corticotrophin releasing factor inhibits cell proliferation and promotes apoptosis through upregulation of tumor protein p53 in human glioma. Oncol Lett 15:8378-8386 (2018). Read more (PubMed: 29805572) »
  • Zheng LL  et al. Preimplantation maternal stress impairs embryo development by inducing oviductal apoptosis with activation of the Fas system. Mol Hum Reprod 22:778-790 (2016). Read more (PubMed: 27475493) »
See all 7 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Zebrafish Tissue sections (retina)
Permeabilization
No
Specification
retina
Blocking step
FBS in PBS as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Mr. Jiaheng Xie

Verified customer

Submitted May 30 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (Intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
14 µg
Specification
Intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 22 2015

Application
Western blot
Sample
Rat Tissue lysate - whole (Brain)
Loading amount
30 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (Gradient)
Blocking step
Casein as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 04 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - Triton-X
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 15 2012

Answer

Thank you for contacting us.

As we discussed on the phone, adding a BSA block and trying a shorter antigen retrieval time may help to reduce the background for ab7963, ab59023, and ab75168, while trying a 1:100 dilution for ab35748 may help to improve your signal. Please let me know how your results turn out. If you these antibodies continue to give poor results, I will be happy to offer you free of charge replacements, credits, or refunds to the antibodies that are guaranteed for use on rat samples. Please let me know your original order or PO numbers and how you would like to proceed and I will be happy to help you further.

Read More

Answer

DISCOUNT CODES :
**** for ab1376
**** for ab59023
Expiration date: DD MM YYYY for both codes

I am very pleased to hear you would like to accept our offer and test ab1376 and ab59023 in Pig.Each code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forPig and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your inquiry.
Unfortunately, this antibody was not tested with samples from a knock out mouse in WB by our lab. We also do not have any data/information about other customers using this antibody in CRF1 ko mice.
I am sorry I did not have a positive answer to your questions on this occasion and wish you best of luck with your publication.

Read More

Answer

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.


I look forward to receiving your reply.

Read More

Answer

I am sorry that you are having difficulties using some of our antibodies in western blot. From the data on the website, it does appear that in addition to recognizing the bands of interest, for ab59023 a 50kDa band and for ab75168, a band at 48kDa, both antibodies do give non-specific bands at various molecular weights. In your western blots, are you seeing a band at the right size and the non-specific bands or just the non-specific bands? For ab59023, the epitope region is between ******of the human sequence and for ab75168 the epitope sequence is ******of the human sequence. I would be happy to help with some protocol suggestions, could you provide me with the protocol that you using, blocking conditions, antibody concentration and incubations times etc. I will then see if I can make any recommendations that would help. I look forward to your reply and helping you resolve this issue.

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain slices)
Specification
Brain slices
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Dr. Christoph Schwarzer

Verified customer

Submitted Jan 31 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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