Key features and details
- Goat polyclonal to CRF1/CRHR1
- Suitable for: IHC-P, ELISA, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-CRF1/CRHR1 antibody
See all CRF1/CRHR1 primary antibodies
DescriptionGoat polyclonal to CRF1/CRHR1
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Sheep, Chicken, Human, Pufferfish, Rhesus monkey, African green monkey, Syrian hamster
Synthetic peptide corresponding to Human CRF1/CRHR1 aa 279-292 (internal sequence) (Cysteine residue). Synthetic peptide:
KLYYDNEKCWFGKRby a Cysteine residue linker, corresponding to internal sequence amino acids 279-292 of Human CRF1/CRHR1(Peptide available as ab113546.).
(Peptide available as
This product was previously labelled as CRF1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab59023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionThis is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Tissue specificityPredominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
modificationsC-terminal Ser or Thr residues may be phosphorylated.
Cellular localizationCell membrane.
- Information by UniProt
- Corticotropin releasing factor type 1 receptor antibody
- corticotropin releasing hormone receptor 1 antibody
- Corticotropin-releasing factor receptor 1 antibody
ab59023 (1/100) staining CRF1/CRHR1 in paraffin embedded mouse brain tissue sections. Tissues were fixed in paraformaldehyde and permeabilised with 0.3% Triton X100/TBS. Please refer to abreview for further experimental details.
All lanes : Anti-CRF1/CRHR1 antibody (ab59023) at 1 µg/ml
Lane 1 : Rat Forebrain
Lane 2 : Rat Cortex Tissue Lysate
Lane 3 : Cerebellum Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Additional bands at: 110 kDa, 70 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ab59023 has been referenced in 13 publications.
- Wang Y et al. Corticotropin-releasing factor depolarizes rat lateral vestibular nuclear neurons through activation of CRF receptors 1 and 2. Neuropeptides 76:101934 (2019). PubMed: 31130301
- Savarese A & Lasek AW Regulation of anxiety-like behavior and Crhr1 expression in the basolateral amygdala by LMO3. Psychoneuroendocrinology 92:13-20 (2018). PubMed: 29609111
- Li CY et al. Restraint-induced corticotrophin-releasing hormone elevation triggers apoptosis of ovarian cells and impairs oocyte competence via activation of the Fas/FasL system. Biol Reprod 99:828-837 (2018). PubMed: 29668880
- Feng Y et al. Human corticotrophin releasing factor inhibits cell proliferation and promotes apoptosis through upregulation of tumor protein p53 in human glioma. Oncol Lett 15:8378-8386 (2018). PubMed: 29805572
- Chen H et al. Activation of corticotropin-releasing factor receptor 1 aggravates dextran sodium sulphate-induced colitis in mice by promoting M1 macrophage polarization. Mol Med Rep 17:234-242 (2018). PubMed: 29115460