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I've been trying to optimise the goat polyclonal antibody to CRF1ab59023 on paraffin sections of mouse tissues, where I would expect tosee some expression.
I took 1/100 as a starting point, as that is the recommended dilutionin the data sheet and used antigen retrieval in pressure cooker and ananti-goat biotinylated secondary - streptavidin-peroxidase with DABdetection. So far I cannot see any staining.
Please can you suggestanything to help.
At the same time I have been using the rabbit polyclonal to CRFreceptor 2 (ab75168) on the same tissues and have had good specificpositive staining.
Many thanks for your help.
Asked on Feb 08 2012
Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
I look forward to receiving your reply.
Answered on Feb 08 2012