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I have purchased one of your antibodies against CRFR1 (ab59023) and used it for western blog analysis. I was now asked by reviewers of a paper i submitted using this antibody to show that it has specificity for CRFR1. I would like to know if you did any western blot control for this using knockout/knockdown tissue.
Asked on May 07 2012
Thank you for your inquiry.
Unfortunately, this antibody was not tested with samples from a knock out mouse in WB by our lab. We also do not have any data/information about other customers using this antibody in CRF1 ko mice.
I am sorry I did not have a positive answer to your questions on this occasion and wish you best of luck with your publication.
Answered on May 07 2012