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ab35748 gives no signal and ab7963, ab59023, and ab75168 give high background in IHC-P on rat samples.
Asked on Aug 03 2012
Thank you for contacting us.
As we discussed on the phone, adding a BSA block and trying a shorter antigen retrieval time may help to reduce the background for ab7963, ab59023, and ab75168, while trying a 1:100 dilution for ab35748 may help to improve your signal. Please let me know how your results turn out. If you these antibodies continue to give poor results, I will be happy to offer you free of charge replacements, credits, or refunds to the antibodies that are guaranteed for use on rat samples. Please let me know your original order or PO numbers and how you would like to proceed and I will be happy to help you further.
Answered on Aug 03 2012