Product nameAnti-CRHR2 antibody
See all CRHR2 primary antibodies
DescriptionRabbit polyclonal to CRHR2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Horse, Hamster, Cow, Dog, Human
Synthetic peptide corresponding to Rat CRHR2 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.
This product was previously labelled as Corticotropin Releasing Factor Receptor 2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab104368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).|
FunctionThis is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
Cellular localizationCell membrane.
- Information by UniProt
- Corticotropin releasing hormone receptor 2 antibody
- Corticotropin-releasing factor receptor 2 antibody
- Corticotropin-releasing hormone receptor 2 antibody
All lanes : Anti-CRHR2 antibody (ab104368) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Rat Forebrain
Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 51 + 54 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
CRHR2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.
ab104368 has not yet been referenced specifically in any publications.