Key features and details
- Rabbit polyclonal to CRHR2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CRHR2 antibody
See all CRHR2 primary antibodies
DescriptionRabbit polyclonal to CRHR2
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human CRHR2 aa 20-108.
Database link: Q13324
- IHC-P: Human adrenal gland tissue. ICC/IF: HepG2 cells. WB: Mouse and Rat brain.
This product was previously labelled as Corticotropin Releasing Factor Receptor 2
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236982 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||Use a concentration of 3 µg/ml. Predicted molecular weight: 48 kDa.|
FunctionThis is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
Cellular localizationCell membrane.
- Information by UniProt
- Corticotropin releasing hormone receptor 2 antibody
- Corticotropin-releasing factor receptor 2 antibody
- Corticotropin-releasing hormone receptor 2 antibody
Paraffin-embedded human adrenal gland tissue stained for CRHR2 with ab236982 at a 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-CRHR2 antibody (ab236982) at 3 µg/ml
Lane 1 : Rat brain tissue
Lane 2 : Mouse brain tissue
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 48 kDa
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for CRHR2 using ab236982 at a dilution of 1/100 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236982 has not yet been referenced specifically in any publications.