Product nameAnti-Cripto1 antibody
See all Cripto1 primary antibodies
DescriptionRabbit polyclonal to Cripto1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Cow
- Human Embryonic Stem Cells, Cripto1 Recombinant Protein
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa).|
FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Sequence similaritiesContains 1 EGF-like domain.
Cellular localizationCell membrane.
- Information by UniProt
- CR antibody
- CRGF antibody
- Cripto 1 antibody
All lanes : Anti-Cripto1 antibody (ab19917) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate at 20 µg
Lane 2 : Cripto protein at 0.1 µg
Lane 3 : Human Embryonic Stem Cell Lysate at 20 µg with Human Cripto1 peptide (ab23310) at 1 µg/ml
Lane 4 : Cripto protein at 0.1 µg with Human Cripto1 peptide (ab23310) at 1 µg/ml
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 21 kDa
The 17 kDa band detected in human embryonic stem cells may correspond with Cripto that has had its signal sequence cleaved. The Cripto bands were blocked with the immunizing peptide, ab23310.
ICC/IF image of ab19917 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Anti-Cripto antibody, ab19917, stained the membrane of human embryonic stem cells at a dilution of 1/100.
The image shows staining of human pancreas tissue. Staining was nuclear in some islet cells and cytoplasmic in some cells of the acini. Antigen retrieval was performed using Citrate at pH6. Nuclear localization of Cripto protein has been described in the literature: Gong YP et al. Eur J Surg Oncol, 2006.
This product has been referenced in:
- Lo RC et al. Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/ß-catenin pathway. Cell Death Differ 25:1426-1441 (2018). Read more (PubMed: 29445127) »
- Liu Q et al. Cripto-1 acts as a functional marker of cancer stem-like cells and predicts prognosis of the patients in esophageal squamous cell carcinoma. Mol Cancer 16:81 (2017). Read more (PubMed: 28431580) »