Key features and details
- Rabbit polyclonal to Cripto1/CRIPTO
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cripto1/CRIPTO antibody
See all Cripto1/CRIPTO primary antibodies
DescriptionRabbit polyclonal to Cripto1/CRIPTO
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
- MDA-MB231 lysate; HeLa and NTERA-2 cells.
This product was previously labelled as Cripto1
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.88% Sodium chloride, 99% Tris glycine
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab178560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 21 kDa.|
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/20 - 1/100.|
FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Sequence similaritiesContains 1 EGF-like domain.
Cellular localizationCell membrane.
- Information by UniProt
- CR antibody
- CRGF antibody
- Cripto 1 antibody
Flow cytometric analysis of NTERA-2 cells labeling Cripto1/CRIPTO with ab178560 at 1/50 dilution, detected using Dylight-488 conjugated goat anti-rabbit IgG secondary.
Anti-Cripto1/CRIPTO antibody (ab178560) at 1/1000 dilution + MDA-MB231 lysate
Predicted band size: 21 kDa
Immunofluorescent analysis of HeLa cells labeling Cripto1/CRIPTO with ab178560 at 1/20 dilution, with Dylight 488 (green). Nuclei were counterstained with DAPI (blue) and tubulin was stained with alpha tubulin (red).
ab178560 has not yet been referenced specifically in any publications.