Overview

  • Product name

    Anti-Cripto1/CRIPTO antibody
    See all Cripto1/CRIPTO primary antibodies
  • Description

    Rabbit polyclonal to Cripto1/CRIPTO
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Human Cripto1/CRIPTO aa 50-150 (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab23309, ab23310)

    Synthetic peptide corresponding to Human Cripto1/CRIPTO aa 100 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab23309, ab23310)

  • Positive control

    • Human Embryonic Stem Cells, Cripto1/CRIPTO Recombinant Protein
  • General notes

     This product was previously labelled as Cripto1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab19917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • Tissue specificity

    Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CR antibody
    • CRGF antibody
    • Cripto 1 antibody
    • Cripto 1 growth factor antibody
    • cripto antibody
    • Cripto-1 growth factor antibody
    • Cripto1 growth factor antibody
    • Epidermal growth factor like cripto protein CR1 antibody
    • Epidermal growth factor-like cripto protein CR1 antibody
    • TDGF 1 antibody
    • TDGF1 antibody
    • TDGF1_HUMAN antibody
    • Teratocarcinoma derived growth factor 1 antibody
    • Teratocarcinoma-derived growth factor 1 antibody
    see all

Images

  • All lanes : Anti-Cripto1/CRIPTO antibody (ab19917) at 1 µg/ml

    Lane 1 : Human Embryonic Stem Cell Lysate at 20 µg
    Lane 2 : Cripto1/CRIPTO protein at 0.1 µg
    Lane 3 : Human Embryonic Stem Cell Lysate at 20 µg with Human Cripto1/CRIPTO peptide at 1 µg/ml
    Lane 4 : Cripto1/CRIPTO protein at 0.1 µg with Human Cripto1/CRIPTO peptide at 1 µg/ml

    Secondary
    All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

    Predicted band size: 21 kDa



    The 17 kDa band detected in human embryonic stem cells may correspond with Cripto1/CRIPTO that has had its signal sequence cleaved. The Cripto1/CRIPTO bands were blocked with the immunizing peptide, ab23310.

  • ICC/IF image of ab19917 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19917, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Anti-Cripto1/CRIPTO antibody, ab19917, stained the membrane of human embryonic stem cells at a dilution of 1/100.

  • The image shows staining of human pancreas tissue. Staining was nuclear in some islet cells and cytoplasmic in some cells of the acini. Antigen retrieval was performed using Citrate at pH6. Nuclear localization of Cripto1/CRIPTO protein has been described in the literature: Gong YP et al. Eur J Surg Oncol, 2006.

References

This product has been referenced in:

  • Gudbergsson JM & Duroux M An evaluation of different Cripto-1 antibodies and their variable results. J Cell Biochem N/A:N/A (2019). Read more (PubMed: 31310365) »
  • Lo RC  et al. Cripto-1 contributes to stemness in hepatocellular carcinoma by stabilizing Dishevelled-3 and activating Wnt/ß-catenin pathway. Cell Death Differ 25:1426-1441 (2018). Read more (PubMed: 29445127) »
See all 11 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (induced human iPSCs)
Specification
induced human iPSCs
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 17 2019

Answer

Thank you for your message.

I am sorry we do not sell the human embryonic stem cells.

As far as we are aware, this antibody has not been tested in other sample types. I can recommend the customer try a literature search to obtain further information on tissues that Cripto1 is expressed in. For example, the following link provides information from the
Unigen EST expression profile for the protein. I hope this will be helpful.

http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.385870

Some of our other Cripto1 antibodies have been used successfullyin NCCIT cells, 181 (Human embryonic stem cell line).

I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

Read More

Answer

Thank you for contacting us.

I am afraid we don’t have any information about the use of this antibody in Functional Assays.

However, if you are interested in participating in our antibodies evaluation system (Abreview), and test any of these antibodies in these assays, I will be happy to offer a discount code for a future purchase if you submit the results (in a form of an Abreview) to our website.

Therefore, I can offer a discount off a future purchase if you buy an antibody / ies and test it / them in a new application and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test the antibody / ies in a concrete assay. I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

2. Purchase the antibody either by phone, fax, or online (www.abcam.com).

3. Test it in the new application.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!).

To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: http://ops.adminsite.com/admin/crm2/www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

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