Recombinant
RabMAb

Recombinant Anti-Cripto1/CRIPTO antibody [EPNCI106B] (ab133236)

Overview

  • Product name

    Anti-Cripto1/CRIPTO antibody [EPNCI106B]
    See all Cripto1/CRIPTO primary antibodies
  • Description

    Rabbit monoclonal [EPNCI106B] to Cripto1/CRIPTO
  • Host species

    Rabbit
  • Specificity

    ab133236 cross reacts with Cripto 3.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    corresponding to Human Cripto1/CRIPTO.
    Database link: P13385
    (Peptide available as ab193608)

  • Positive control

    • NCCIT and F9 cell lysates
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product was previously labelled as Cripto1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.Can be blocked with Cripto1/CRIPTO peptide (ab193608).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
    • Tissue specificity

      Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
    • Sequence similarities

      Contains 1 EGF-like domain.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • CR antibody
      • CRGF antibody
      • Cripto 1 antibody
      • Cripto 1 growth factor antibody
      • cripto antibody
      • Cripto-1 growth factor antibody
      • Cripto1 growth factor antibody
      • Epidermal growth factor like cripto protein CR1 antibody
      • Epidermal growth factor-like cripto protein CR1 antibody
      • TDGF 1 antibody
      • TDGF1 antibody
      • TDGF1_HUMAN antibody
      • Teratocarcinoma derived growth factor 1 antibody
      • Teratocarcinoma-derived growth factor 1 antibody
      see all

    Images

    • All lanes : Anti-Cripto1/CRIPTO antibody [EPNCI106B] (ab133236) at 1/1000 dilution

      Lane 1 : NCCIT lysates
      Lane 2 : F9 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 21 kDa

    References

    This product has been referenced in:

    • Park SW  et al. The expression of the embryonic gene Cripto-1 is regulated by OCT4 in human embryonal carcinoma NCCIT cells. FEBS Lett 592:24-35 (2018). Read more (PubMed: 29223130) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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