Recombinant Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP)
See all Cripto1/CRIPTO primary antibodies -
Description
Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO (HRP) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cripto1/CRIPTO. The exact sequence is proprietary.
(Peptide available asab193609) -
Positive control
- WB: NCCIT whole cell lysate
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.
This product was previously labelled as Cripto1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR106A -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
Tissue specificity
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6997 Human
- Omim: 187395 Human
- SwissProt: P13385 Human
- Unigene: 385870 Human
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Alternative names
- CR antibody
- CRGF antibody
- Cripto 1 antibody
see all
Images
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![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)](https://www.abcam.com/ps/products/203/ab203468/Images/ab203468-248700-WBab2034681.jpg)
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468) at 1/5000 dilution + NCCIT (Human embryonic teratocarcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203468 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Protocols
References
ab203468 has not yet been referenced specifically in any publications.
