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  1. Link

    cripto1cripto-antibody-epncir106a-hrp-ab203468.pdf

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Stem Cells Lineage Markers Ectoderm
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Recombinant
RabMAb

Recombinant Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)

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  • Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP)
    See all Cripto1/CRIPTO primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cripto1/CRIPTO. The exact sequence is proprietary.
    (Peptide available as ab193609)

  • Positive control

    • WB: NCCIT whole cell lysate
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.

    This product was previously labelled as Cripto1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Proclin
    Constituents: 30% Glycerol, 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR106A
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Embryonic Stem Cells
    • Secreted
    • Developmental Biology
    • Embryogenesis
    • Morphogens
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Alternative Versions

    • Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391)
    • Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (Allophycocyanin) (ab225076)
    • Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (Phycoerythrin) (ab225077)
    • Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (HRP) (ab199507)
  • Recombinant Protein

    • Recombinant human Cripto1/CRIPTO protein (Fc Chimera) (ab84062)

Applications

Our Abpromise guarantee covers the use of ab203468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).

Target

  • Function

    Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • Tissue specificity

    Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P13385 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6997 Human
    • Omim: 187395 Human
    • SwissProt: P13385 Human
    • Unigene: 385870 Human
    • Alternative names

      • CR antibody
      • CRGF antibody
      • Cripto 1 antibody
      • Cripto 1 growth factor antibody
      • cripto antibody
      • Cripto-1 growth factor antibody
      • Cripto1 growth factor antibody
      • Epidermal growth factor like cripto protein CR1 antibody
      • Epidermal growth factor-like cripto protein CR1 antibody
      • TDGF 1 antibody
      • TDGF1 antibody
      • TDGF1_HUMAN antibody
      • Teratocarcinoma derived growth factor 1 antibody
      • Teratocarcinoma-derived growth factor 1 antibody
      see all

    Images

    • Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)
      Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)
      Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468) at 1/5000 dilution + NCCIT (Human embryonic teratocarcinoma cell line) Whole Cell Lysate at 10 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 21 kDa
      Observed band size: 24 kDa
      why is the actual band size different from the predicted?


      Exposure time: 12 minutes


      This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203468 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

       

       

    Protocols

    • Western blot protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References

    ab203468 has not yet been referenced specifically in any publications.

    Publishing research using ab203468? Please let us know so that we can cite the reference in this datasheet.

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