Product nameAnti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP)
See all Cripto1/CRIPTO primary antibodies
DescriptionRabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Cripto1/CRIPTO. The exact sequence is proprietary.
(Peptide available as
- WB: NCCIT whole cell lysate
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.
This product was previously labelled as Cripto1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).|
FunctionCould play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
Tissue specificityPreferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
Sequence similaritiesContains 1 EGF-like domain.
Cellular localizationCell membrane.
- Information by UniProt
- CR antibody
- CRGF antibody
- Cripto 1 antibody
Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468) at 1/5000 dilution + NCCIT (Human embryonic teratocarcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203468 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab203468 has not yet been referenced specifically in any publications.