Recombinant Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO (HRP)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Overview
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Product name
Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP)
See all Cripto1/CRIPTO primary antibodies -
Description
Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO (HRP) -
Host species
Rabbit -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Cripto1/CRIPTO. The exact sequence is proprietary.
(Peptide available asab193609) -
Positive control
- WB: NCCIT whole cell lysate
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.
This product was previously labelled as Cripto1.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol, 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR106A -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab203468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa). |
Target
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Function
Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm. -
Tissue specificity
Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 6997 Human
- Omim: 187395 Human
- SwissProt: P13385 Human
- Unigene: 385870 Human
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Alternative names
- CR antibody
- CRGF antibody
- Cripto 1 antibody
see all
Images
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![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)](https://www.abcam.com/ps/products/203/ab203468/Images/ab203468-248700-WBab2034681.jpg)
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468) at 1/5000 dilution + NCCIT (Human embryonic teratocarcinoma cell line) Whole Cell Lysate at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 12 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203468 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
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![Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)](https://www.abcam.com/ps/products/203/ab203468/Images/ab203468-3-benefits-of-recombinant-antibodies.png)
Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (HRP) (ab203468)
References (0)
ab203468 has not yet been referenced specifically in any publications.
