Recombinant
RabMAb

Recombinant Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (Phycoerythrin) (ab225077)

Overview

  • Product name

    Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (Phycoerythrin)
    See all Cripto1/CRIPTO primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cripto1/CRIPTO. The exact sequence is proprietary.
    Database link: P13385
    (Peptide available as ab193609)

  • Positive control

    • Flow Cyt: NCCIT cells
  • General notes

     This product was previously labelled as Cripto1

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab225077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1000.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
  • Tissue specificity

    Preferentially expressed in gastric and colorectal carcinomas than in their normal counterparts.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CR antibody
    • CRGF antibody
    • Cripto 1 antibody
    • Cripto 1 growth factor antibody
    • cripto antibody
    • Cripto-1 growth factor antibody
    • Cripto1 growth factor antibody
    • Epidermal growth factor like cripto protein CR1 antibody
    • Epidermal growth factor-like cripto protein CR1 antibody
    • TDGF 1 antibody
    • TDGF1 antibody
    • TDGF1_HUMAN antibody
    • Teratocarcinoma derived growth factor 1 antibody
    • Teratocarcinoma-derived growth factor 1 antibody
    see all

Images

  • Overlay histogram showing NCCIT cells stained with ab225077 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225077, 1/1000 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in NCCIT cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab225077 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225077.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up